Scientific. 2008 4 Journal of Kerbala University , Vol. 6 No. 117 Study the anti-Bacterial &Fungal activity of Junchus Arabica on Some Pathogenic isolates Invitro Ali Muknes*,Mahdi Murshd**,Mohammed Abdul-Mounther***,Abdulla Khadam**** *Asst. Prof.Macrobiolo.Basrah Tech.Instut,** Msc.Path.College of medicine, Thi-Qar Univ.Iraq, ***Ph.D.Biochem.College of medicine, Thi-Qar Univ.Iraq,**** Asst. Prof.Macrobiolo.Basrah Tech.Instut. Abstract : To demonstrate and investigate in vitro the activity of antibacterial and fungal of fao junchus Arabica fresh and dry seeds obtained from different areas of Basrah especially against (5)isolates of pathogenic bacteria aerobic Escherichia coli, Pseudomonas aeruginosa klebsiella spp., Staphylococcus aureus and anaerobic bacteria Propinipionibacterium acnesisolated from different skin diseases as well as antifungal activity against (5) Tinea corporis , Tinea pedis, Tinea unquium , Candida albicans ,Tinea cruris dermophytes of skin- hair and nail besides candida albicans.Green and dry seeds showed high activity effect of both antibacterial and fungal. خلصة : ال اسحفظح انثصشج،نذس يحا فيفح انري ذسرىطنجا انخضشاء وا)سهيان( ثاخ ثاذيح نثزوسخ انسرخهصا اسرخذيد ان ( نفطشياخ ضذ نهجشاثيى واضادجان فعانيرها5 شضح يخرهفح نشضيح ي إصاتاخ جهذيح يعزونح يي عزلخ جشثىي) اجعىا يسرشف سهد انعزلخثاب، ش ذسثة حة انششضيح عزلخ ينعاو،إضافح إن انثصشج اEscherichia coli, pseudomonas aeruginosa , Klebsiella spp., Staphylococcus aureus وانعزنح انلهىائيح Propinipionibacterium acnes ( واسرخذيدا ك5 ح يثم عزلخ فطشيح جهذي) Tinea cruris, Tinea corporis , Tinea pedis, Tinea unquium  إضافح إنCandida albicans نفعانيحل اازنك ذى اسرع ك ( ضذ3 نقياسيح انجشاثيى اىاع يأ) Staphylococcus ureus ATCC29213 Pseudomonas aeruginosa ATCC27853 , Escherichia coli ATCC25922 ثيطيسم فعانيح ذثخ انثا أظهشخ تزوس ح نكل انجشاثيى زنك أظهشخسم أيضا. كرخهص تزوس انح يس ذأثشخ تفعانيح انرينقياسينجشاثيى ارها تاذ يقاسسرخذيح عخ اننفطشيا وا سم تزوس ان يشاته إنسرىيثيضاخ وت اننجهذيح إضافح إنخ انفطشيانيح ضذ انجشاثيى وانجافح فعاسم اخ انثا تزوس سم انخ انثا  أ انخضشاء. فصائمح يشضي يضاد نعزلخ ي راخ يفعىل)ىب انعشاقج( في انثصشج ري ذسرىط خرهفح .شضيح ان انجشاثيى ان Introduction In the past peoples as its innate information were used many plant source as treatment of some diseases or others complication of this diseases, but they were not know the complication or side effects and not able to demonstrated this plants drugs, the aims of using this plant materials and its derivations was to recovery or healing the patients and fifty percent of pharmaceuticals are derived from the plants. All of plant are useful and rich in wide variety of secondary metabolites :such as tannins,terpenoids :alkaloid,and flavonoids (1) , and have applied as antibacterial or antivirus (2) an aqueous prepation of this plant is used as acosmetic and medicine (3,4,5) . Materials and Methods Green seed and dry seeds of Junchus Arabica had obtained from different regions of Basrah city. All of sample of this plant culture in Basrah especially in different region samples of were crushed with a pestle and mortar 50g of seeds added to 250 mL ethanol for three days with intermittent agitation . The mixture was filtered and crude extract was collected and then distilled at 37Cº using in an oven.The crude plant extract was diluted into (25,12.5,6.35,3.12,1.56,0.78mL) and finally 0.39mL by using distilled water (sterile).Ten