International Journal of Latest Technology in Engineering & Management (IJLTEM) ISSN: 2456-1770 www.ijltem.com Volume 1 Issue 2 ǁ July. 2016 ǁ PP 16-19 | Volume 1| Issue 2 | www.ijltem.com | July 2016 |Page 16| Anti-implantation effect of Puraria tuberosa in albino rats Dr. Naveen Chandra Khanduri 1 , Dr. S. P. Singh 1 Department of Zoology, Government Post Graduate College Agastyamuni, Rudraprayag 2 Retd. Reproductive Biology Cell, Department of Zoology, DBS (PG) College, Dehradun Abstract : The folklore medicines of primitive civilization frequently include a large number of plants said to be potent contraceptive and abortfacients. Some of these plants might have been included among folklore medicine as a result of trial and error observations, while for others there may be no reasonable basis. Population hazard in Indian has not only created enormous problem but has ceased our developments and also is the biggest threat to our environment and natural resources. Abrupt rise is the cause of abrupt downfall. According to this phenomenon the sudden burst in population may cause threat to the existence of mankind. This universal problem is not merely understood by the people throughout the world but activated too. Several means and methods have been evolved to control the conceptions. I. Material and Methods: The fresh, air dried, powdered seeds, filtered through muslin clothes were use at 25, 50 and 75 mg/kg/day/rat doses. Each dose along with 10% gum acacia powder was suspended in the distilled water. The volume was adjusted in such a way that 1 ml of suspension corresponds to each dose. Adult, cyclic female albino rats (100- 150 gm) were divided into four groups each with five animals. They were maintained under uniform laboratory conditions with free access of food (Hindustan Liver Ltd.) and tap water. The seed powder as aqueous solution was administered orally by an intregastric catheter. The first group served as control in which 1ml of gum acacia (10%) was administered. In II, III and IV groups 25, 50 and 75 mg/kg dose administered to each rat respectively for 30 days. Before the start of experiments, the body weight of each rat was recorded. On day 31st the rats of groups I, III, IV and V groups were weighed and killed under chloroform anaesthesia. The rats were dissected and genital organs were taken out the weight of ovaries and uterus was also noted. The female rats of groups II, III and IV were mated with normal male rats (1:2) during night hours next morning, the vaginal smear of each female rat was examined for presence of spermatozoa. The day on which the spermatozoa were found in the smear was considered as the 1st day of pregnancy. On the 10th day of pregnancy the rats were laparotomized to know the presence of implantation in the horns of uteri. Anti- implantation: For anti implantation study, mature female rats with regular oestrous cycle were left overnight with mature male rats (3:1). Next morning the vaginal smear of female rats were examined for the presence of spermatozoa. Presence of spermatozoa was treated as the evidence of successful mating. The first day of the pregnancy is assigned on the day when spermatozoa were found in vaginal smear. A dose of 25 mg/kg, 50 mg/kg and 75 mg/kg body weight were given from the first day (D1) upto 7th day (D7). The same procedure repeated to all groups of rats. After 7 days of administration of a dose and 3 days of rest, on the day tenth animals were laparotomized. The horn of uterus was examined for the presence or absence of implants. If implants are not present, it indicates the anti-implantation and anti-fertility effect of the plant. Anti-ovulation : The plant material was administered orally daily for 15 days to female rats at different level i.e. 25 mg, 50 mg, and 75 mg /kg/rat. After 15 days of administration of doses, rats were killed under chloroform anaesthesia and reproductive organs were removed. The ovaries were examined under the bio- nuclear microscope for the presence or absence of corpora-lutea (red spots or bleeding points) and their number were counted. The numbers of corpora-lutea were compared with control female rats. The normal control group were given only the vehicle (10% gum acasia powder in water) for same duration as in experimental rats. Histological slides of the ovaries of above treatments were also observed to note the corpora-lutea. It was concluded that a dose has an anti-ovulatory effect, if the corpora-lutea are lesser in treated group than the control group. The results were presented in the tabular form.