20 This article can be downloaded from http://www.iajavs.com/currentissue.php Indo-Am. J. Agric. & Vet. Sci., 2016 Mohsen Hesami and Mohammad Hossein Daneshvar, 2016 REGENERATION FROM CALLUS WHICH IS PRODUCED FROM COTYLEDON OF ANTIRRHINUM MAJUS Mohsen Hesami 1 and Mohammad Hossein Daneshvar 1 * Antirrhinum majus L. (Snapdragon) is a herbaceous ornamental plant which is cultivated for its attractive view. In this study, three experiments included callus induction, regeneration from callus and in vitro rooting were carried out as a complete randomized design in 3 replications (10 samples). The maximum callus induction frequency (90%) from cotyledon explant was observed on MS medium supplemented with 1.0 mg/L 2,4-D along with 0.1 mg/L BAP. The highest regeneration frequency (93.33%) followed by maximum number of multiple shoots (2.96) as well as length (2.43 cm) were obtained on MS medium supplemented with 1.0 mg/l BAP along with 0.1 mg/L IBA. One hundred percent of the microshoots were rooted on MS medium supplemented with 2.0 mg/L IBA or NAA. A total of 85% survival was achieved when rooted explants were acclimatized ex vitro using 1 perlite: 1 cocopeat mixture. Keywords: Acclimatization, Callus induction, Regeneration, Rooting, Snapdragon *Corresponding Author: Mohammad Hossein Daneshvar  mhdaneshvar2004@yahoo.com INTRODUCTION Antirrhinum majus (snapdragon) is a species of flowering plant belonging to the Scrophulariaceae family. It is native to the Mediterranean region, from Morocco and Portugal north to southern France, and east to Turkey and Syria (Oyama and Baum, 2004). The common name “snapdragon”, originates from the flowers reaction to having their throats squeezed, which causes the “mouth” of the flower to snap open like a dragon’s mouth (Tank et al., 2006). Snapdragon if propagated by seeds cannot ensure genetic uniformity, may produce 1 Department of Horticulture science, Ramin University of Agriculture and Natural Resources, Khoozestan, Iran. Indo-Am. J. Agric. & Vet. Sci., 2016 ISSN 2321–9602 www.iajavs.com Vol. 4, No. 1, February 2016 © 2016 Meghana Publications. All Rights Reserved Research Paper undesired phenotypes and subsequently influence the quality and regeneration potential of the plants, as the plants may be randomly selected without taking necessary care. This will result in negative economic implications on mass production of this plant (Sheyab et al., 2010). Thus, the development of in vitro culture techniques would increase the knowledge about this germplasm with potential relevance as an ornamental crop. So far, a few studies has been done on in vitro propagation of Antirrhinum majus L. (Sangwan and Harada 1975; Newbury 1986; Atkinson et al. 1988; Okubo et al., 1991; Sheyab et al., 2010). Therefore, the aim of this work was to establish