Ahmed et al., J Adv Biotechnol Exp Ther. 2019; 2(3): 140-145 www.bsmiab.org/jabet 140 Phytochemical profiling and antioxidant potentiality of medicinal plants along with their antibacterial efficacy Sheikh Rashel Ahmed 1,2 , Ismat Jahan Romi 1 , Jamil Ahmed 1,3 , Mahmudul Hasan 1,4 , Rubel Roy 1 , Mohammad Mehedi Hasan Khan 1,3 1 Faculty of Biotechnology and Genetic Engineering, Sylhet Agricultural University, Sylhet-3100, Bangladesh. 2 Department of Plant and Environmental Biotechnology, Sylhet Agricultural University, Sylhet-3100, Bangladesh. 3 Department of Biochemistry and Chemistry, Sylhet Agricultural University, Sylhet-3100, Bangladesh. 4 Department of Pharmaceuticals and Industrial Biotechnology, Sylhet Agricultural University, Sylhet-3100, Bangladesh. * Correspondence: Mohammad Mehedi Hasan Khan, Department of Biochemistry and Chemistry, Sylhet Agricultural University, Sylhet- 3100, Bangladesh. E-mail: mehedi2001bdbd@gmail.com, Tel.: +8801680592166 Academic Editor: Dr. Md. Atikur Rahman, National Institute of Animal Science, South Korea Received: 15 July 2019; Accepted: 18 September 2019; Published: 25 September 2019. ABSTRACT: The aim of this study was to explore phytochemical profiling, antioxidant and antibacterial activity of four medicinal plants including Catharanthus roseus, Aegle marmelos, Moringa oleifera, and Ageratum conyzoids grown in Sylhet district, Bangladesh. In this study, total 11 phytochemicals were screened from methanol extract of four medicinal plants, wherein flavonoid, tannin, sterol, phenol were present in all four medicinal plants. In vitro, antioxidant activity of these medicinal plants extract was investigated by DPPH-radical scavenging assay. The Aegle marmelos exhibited the highest antioxidant activity followed by Moringa oleifera, Ageratum conyzoids, and Catharanthus roseus extract. Methanolic extracts of same medicinal plants were subjected to a test of their antibacterial activities against Staphylococcus aureus, Escherichia coli, Klebsiella sp., Pseudomonas sp .and Salmonella sp. by agar disc diffusion method. The highest antibacterial potential was observed in the extract of Aegle marmelos against Salmonella sp. followed by Catharanthus roseus against Pseudomonas sp .with zone of inhibition of 18.67 mm, 15.0 mm, respectively. This study confirmed the efficacy of some native medicinal plants extract as potential source of phytochemicals, along with natural antioxidant and antimicrobials, which provide new possibilities to employing them against disease causing test organisms. KEYWORDS: Methanol, phytochemicals, DPPH, extract. INTRODUCTION Natural products have been a part and parcel of phytomedicines to treat diseases subsequently from time ancient [1]. Medicinal plants are a big source for a wide range of chemical ingredients as drug candidate [2]. The World Health Organization (WHO) estimates that 65-80% of the people relied upon traditional medicine, notable from plant origin, to combat diverse ailments [3]. Medicinal plants possess some organic compounds which influence certain physiological action on the human body and these bioactive substances include alkaloids, tannins, carbohydrates, terpenoids, steroids and flavonoids [4]. These compounds are synthesized in living organisms by means of primary or rather secondary metabolism. Bioactive compounds derived from plants exhibit various biological activities. They are widely used in many areas such as human therapy, agriculture, veterinary, scientific research [5]. Antioxidants play a major role in protecting the biological systems against oxidative stress, which is associated with development of many chronic diseases and disorders [6]. Antioxidants protect our body against free radicals that leads to various pathological conditions such as anaemia, asthma, arthritis, ischemia, inflammation, neurodegeneration etc. It also delays ageing process [7]. Due to the harmful side effect of synthetic antioxidant, the search for safe, nutritional and therapeutic natural antioxidant has increasingly ORIGINAL ARTICLE J Adv Biotechnol Exp Ther. 2019; 2(3): 140-145 eISSN: 2616-4760, https://doi.org/10.5455/jabet.2019.d37 Published by www.bsmiab.org