Novel Compounds in Lyophilized Female Camel Urine
Khogali ME Salwa
1
, Elhassan MA bdalla
2
, Sara A Mohamed
1
and Abdallh EA Barajob
2
1
Central Veterinary Research lab, Alamarat Khartoum, Sudan
2
The National Ribat University, Faculty of Pharmacy, Khartoum, Sudan
*
Corresponding author: Salwa MEK, Department of Biochemistry, Nutrition, Toxicology and Pharmacology, Central Veterinary Research Laboratory, Sudan, Tel:
916742300134; E-mail: salwamuhamed@hotmail.com
Received date: February 25, 2016; Accepted date: October 13, 2016; Published date: October 17, 2016
Copyright: © 2016 Salwa MEK, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted
use, distribution, and reproduction in any medium, provided the original author and source are credited.
Abstract
The present work was done to emphasize the degraded compounds of camel urine. Lyophilized camel urine was
analyzed by Gas chromatography mass spectrometry (GC-MS) Agilent 6890 GC. Eleven bioactive compounds were
detected, four of these compounds have a novel structure, and these compounds are: 4-Heptanone,3-methyl,
Butanoic Acid,butyl ester, Acetic Acid, (2,4,6-triethybenzoyl)thio, Benzoic Acid,methyl ester, Propane,2,2’-
(methylenebis(oxy)bis(2-methyl-), Butane, 1,1-dibutoxy-, Pentanoic Acid, 4-oxo-, butyl ester, Benzoic Acid, butyl
ester, Benzeneacetic Acid, 2-methylpropyl ester, Butylparaben. The four compounds of novel structure are:
Titanium,(08-1,3,5,7-cyclooctatetraene)(05-2,4-cyclopentadien-1-yl),Nitroxidebis(1-1 dimethyleyl), 9,12,15-
octadecatriaenoic acid, Hepta siloxane.
Keywords: Camel urine; Novel compounds; Gas chromatography
Introduction
Urine is a purifed sterile product of blood fltration, medically
referred to as plasma ultra-fltrate made by kidneys [1]. It has been
shown throughout the history of medical science to this day, that urine
has profound medical uses [2].
Use of animal urine is endorsed in mainstream modern medicine.
Pregnant mare urine is the source of conjugated equine estrogens and
has been marketed for over ffy years as pharmaceutical brand
premarin, “an estrogen treatment for menopausal and pre-menopausal
women” especially postpartum-one of the most prescribed drugs in
United States [1]. It was very recently discovered that adding distilled
cow urine to medicaments increases their efectiveness while
decreasing their side-efects, making anti-cancer, anti-tubercular drug
twenty times more efective and anti- bacterial eight times more
efective (on line document), “urine therapy” is a stable of Ayurveda
remedy [2,3].
Clinical studies on camel urine were recorded [4-10]. Te results of
these experiments proved that camel urine consists of many bioactive
complex compounds, which can acts against bacterial, parasitic,
carcinogenic agents and it has the ability to protect the liver against
toxic agents [11].
Materials and Methods
Urine samples collected from natural grazing animals; then it was
lyophilized using Analytical method.
Analytical method
Gas chromatography-Mass spectrometry (GC-MS) was performed
using Agilent 6890 N network GC system interface, with a 60 m
Agilent fused capillary column, DB-5 ms, 0.25 mm 1-D, 0.25 mm
Film- initial temp 100°C, hold for 2 min, then programmed at 2°C/min
to 300ͦc, isothermal temperature was held for 10 min.
Helium carrier gas, head pressure 9.30 psi, column fow 1 ml/min.
injection temp 300ͦc EL source 230°C, total scan mode was cycled at 2
seconds. 1 ml of the given sample has been diluted with 10 ml of
diethyl chloromethane (DCM), and 1 µ was injected using split less
mode.
Results
Nist library was used for the identifcation of the compounds by
comparison with published retention time of the chromatogram.
Corrected areas % obtained by base line subtraction was used to
calculate the percentage of the compounds within the injected amount.
GC-MS chromatogram of the dichloromethane extract of Camel
urine, showed fourteen peaks indicating the presence of fourteen
compounds.
Retention time Name of compound Molecular formula Molecular weight Peak area %
8.13 Titanium C
13
H
13
Ti 217 1.33
8.84 4-Heptanone, 3-methyl C
8
H
16
O 128 2.3
Journal of Infectious Diseases and
Therapy
Salwa et al., J Infect Dis Ther 2016, 4:5
DOI: 10.4172/2332-0877.1000296
Research Article OMICS International
J Infect Dis Ter, an open access journal
ISSN:2332-0877
Volume 4 • Issue 5 • 1000296