176 Original Article Effect of Grape Seed Extract on Lipid Profile and Expression of Interleukin-6 in Polycystic Ovarian Syndrome Wistar Rat Model Zohreh Salmabadi, M.Sc. 1 , Homa Mohseni Kouchesfahani, Ph.D. 1* , Kazem Parivar, Ph.D. 1 , Latifeh Karimzadeh, M.Sc. 2  'HSDUWPHQW RI$QLPDO %LRORJ\ )DFXOW\ RI %LRORJLFDO 6FLHQFHV .KDUD]PL 8QLYHUVLW\ 7HKUDQ ,UDQ  $QLPDO &HQWHU /DERUDWRULHV DQG &HOOXODU DQG 0ROHFXODU 5HVHDUFK /DERUDWRU\ )DFXOW\ RI %LRORJLFDO 6FLHQFHV .KDUD]PL 8QLYHUVLW\ 7HKUDQ ,UDQ Abstract %DFNJURXQG Polycystic ovary syndrome (PCOS) is a common but complex endocrine disorder and is the major cause of anovulation and consequent subfertility. In this study the effect of grape seed extract (GSE) on triglyceride (TG), total cholesterol (TC), high- density lipoprotein-cholestrol (HDL-C), low-density lipoprotein-cholestrol (LDL-C) and interleukin-6 (IL-6) in PCOS Wistar rats were assessed. 0DWHULDOV DQG 0HWKRGV In this experimental study, 84 adult female Wistar rats were divided into 7 groups (n=12) including control (intact), Sham (estradiol valerate solvent injection), control PCOS and 4 experimental PCOS groups. To induce the syndrome, a single subcutaneous injection of 2 mg estradiol valerate was applied. In experimental groups, PCOS rats were treated with different doses of 50, 75, 100 and 200 mg/kg body weight (BW) GSE by intraperitoneal injection for 10 consecutive days. After harvest- ing blood serum, TG was measured by Glycerol-3-phosphate Oxidase-Peoxidase (GPO- PAP), TC by Cholesterol Oxidase-Peroxidase (CHOD-PAP), and HDL-C by sedimenta- tion method, LDL-C by Friedwald calculation and IL-6 by ELISA method. The serum YDOXHV RI HDFK SDUDPHWHU ZHUH DQDO\]HG XVLQJ RQHZD\ $129$ DW 3 5HVXOWV ,Q DOO H[SHULPHQWDO JURXSV VLJQLｿFDQW GHFUHDVH RI YLVFHUDO IDW ZDV REYLRXV DV compared with control PCOS group. LDL-C, TC and IL-6 levels in experimental groups, SDUWLFXODUO\ DW GRVH RI  PJNJ RI *6( ZHUH VLJQLｿFDQWO\ GHFUHDVHG DV FRPSDUHG ZLWK 3&26 JURXS +RZHYHU +'/& OHYHOV ZHUH QRW VLJQLｿFDQWO\ FKDQJHG &RQFOXVLRQ  $FFRUGLQJ WR WKH ｿQGLQJV RI WKLV VWXG\ LW FDQ EH FRQFOXGHG WKDW *6( ZLWK its effects on serum TC, LDL-C and IL-6 could reduce the effects of dyslipidemia and LQﾀDPPDWLRQ LQ 3&26 UDWV DQG LPSURYH V\VWHPLF V\PSWRPV RI 3&26 Keywords: Dyslipidemia, Grape Seed Extract, IL-6, Polycystic Ovarian Syndrome, Wistar Rat Citation: Salmabadi Z, Mohseni Kouchesfahani H, Parivar K, Karimzadeh L. Effect of grape seed extract on lipid SURｿOH DQG H[SUHVVLRQ RI LQWHUOHXNLQ LQ SRO\F\VWLF RYDULDQ V\QGURPH ZLVWDU UDW PRGHO ,QW - )HUWLO 6WHULO    GRL LMIV Introduction One of the most common endocrine disorders in women is polycystic ovarian syndrome (PCOS), af- fecting about 5 to 10% of women of reproductive age  WR  \HDUV ROG  3&26 ZDV ｿUVW GHVFULEHG LQ 1935 by Stein and Leventhal (2). PCOS is a hetero- geneous disease with a spectrum of endocrine pro- tests such as polycystic ovarian morphology, ovarian follicular theca cell hyperplasia, chronic anovula- tion, menstrual disturbances and infertility. Common 5HFHLYHG  -XO  $FFHSWHG  2FW  &RUUHVSRQGLQJ $GGUHVV 32%R[  'HSDUWPHQW RI $QL- mal Biology, Faculty of Biological Sciences, Kharazmi University, Tehran, Iran (PDLO NRXFKHVIHKDQL#\DKRRFRP Royan Institute International Journal of Fertility and Sterility 9RO  1R  2FW'HF  3DJHV 