This article has been accepted for publication and undergone full peer review but has not been through the copyediting, typesetting, pagination and proofreading process which may lead to differences between this version and the Version of Record. Please cite this article as doi: 10.1002/mrc.4874 This article is protected by copyright. All rights reserved. Castro-Gamboa Ian (Orcid ID: 0000-0002-2353-0181) Giraudeau Patrick (Orcid ID: 0000-0001-9346-9147) Improvement of Bioactive Metabolite Production in Microbial Cultures - A systems approach by OSMAC and deconvolution-based 1 HNMR quantification A systems approach by OSMAC and deconvolution-based 1 HNMR quantification Denise Medeiros Selegato a , Rafael Teixeira Freire b , Alan César Pilon c , Carolina Rabal Biasetto a , Haroldo Cesar de Oliveira d , Lucas Magalhães de Abreu e , Angela Regina Araujo a , Vanderlan da Silva Bolzani a , Ian Castro-Gamboa a* a Nucleus of Bioassays, Biosynthesis and Ecophysiology of natural products (NuBBE), Organic Chemistry Depart., Institute of Chemistry, São Paulo State University (UNESP), St. Prof. Francisco Degni, 55, Quitandinha, Post code 14800-060, Araraquara, SP, Brazil. b Medway Metabonomics Research Group, University of Greenwich, Post Code ME4 4TB, Chatham Maritime, Kent, UK. c Núcleo de Pesquisa em Produtos Naturais e Sintéticos (NPPNS), Faculdade de Ciências Farmacêuticas, São Paulo University (USP), Avenida do Café s/n, Monte Alegre, Post Code 14040-903, Ribeirão Preto, SP, Brazil. d Laboratório de Micologia Clínica, Núcleo de Proteômica, Faculdade de Ciências Farmacêuticas de Araraquara, São Paulo State University (UNESP), Rodovia Araraquara Jaú, Km 01 - s/n, Campos Ville, Post Code 14800-903, Araraquara, SP, Brazil. e Departamento de Fisiopatologia, Universidade de Viçosa (UFV), Campus Universitário, s/n, Post Code 36570 000, Viçosa, MG, Brazil. *Corresponding author: Dr. Ian Castro-Gamboa. E-mail address: ian.castro@gmail.com. Phone: +55 (16) 3301-9664.