RESEARCH ARTICLE
How to cite this article
Ngongu,I., Ugese, F., Liamngee, K., and
Agatsa, D.T.(2018), Isolation and
Identification of Fungi Causing
Postharvest Decay of Oranges (Citrus
sinensis) During Storage in Makurdi,
Mind Sourcing Bio. 3(12), Pp 8.
Received: November 30, 2018
Accepted: Decemberer 5, 2018
Published:December,31 2018
Copyright
© 2018 Ngongu et al
Corresponding author
Liamngee Kator
katorliamgee@gmail.com
Distributed under
Creative Commons CC-BY 4.0
Isolation and Identification of Fungi
Causing Postharvest Decay of Oranges
(Citrus sinensis) During Storage in Makurdi
1
Ngongu Iordye ,
2
Ugese F.D.,
3
Liamngee Kator and
4
Agatsa David Terna
1
Centre for Food Technology and Research, Benue State University, Makurdi, Nigeria.
2
Department of Crop Production, University of Agriculture, Makurdi, Nigeria.
3,4
Department of Biological Sciences, Benue State University, Makurdi, Nigeria.
ABSTRACT
Isolation and identification of fungi causing post-harvest decay of oranges during
storage in Makurdi was investigated. Orange fruits showing symptoms of decay were
collected in polythene envelops and taken to the botany laboratory of the Benue State
University for isolation and identification of fungi pathogens. Small sizes were cut from
the decaying orange fruits, surface sterilized in 1% of sodium hypochloride solution and
rinsed in three changes of sterile distilled water. The excised sections were placed on
solidified Potato Dextrose Agar (PDA) and observed for fungal growth. For
pathogenicity, healthy orange fruits were plugged with pure cultures of the fungal
isolates and disease incidence and severity were assessed. T-test revealed significant
differences between the inoculated and the control at 5% level of probability in the
pathogenicity of the fungal isolates. Aspergillus niger, Penicillium chrysogenum, Rhizopus
stolonifer, Fusarium oxysporum and Bipolaris harlodes were isolated from the decaying
orange fruits. Incidence of decay on healthy orange fruits was 100% for all fungal isolates
while the control was 0%. Severity of decay ranged from 3 – 5 which indicated 41-100%
of tissue damage. Pathogenic fungi on orange fruits are a potential health hazard to man
and animals following ingestion.
Keywords: Postharvest, oranges, storage, decay, fungi
INTRODUCTION
Orange (Citrus sinensis L.) which belongs to the family Rutaceae is one of the most widely
grown fruit crops in the world [1].The crop is mainly cultivated in the tropical and
subtropical regions of the world in over 137 countries on six continents [2]. It is widely
consumed both as fresh fruit and as juice.
Oranges are principal sources of important nutrients like vitamin C, flavonoids, pectin, folic
acid, carotenoid, dietary fibre, potassium, selenium and a wide range of phytochemicals
that are suggested to be responsible for the prevention of degenerative diseases [1,3].
MIND SOURCING
Biology