Research Article The Registration of a Biomaser-Like Effect in an Enzyme System with an RTM Sensor Yu. D. Ivanov , 1,2 K. A. Malsagova , 1 S. G. Vesnin, 3 V. Yu. Tatur, 4 N. D. Ivanova, 5 and V. S. Ziborov 1,2 1 Institute of Biomedical Chemistry (IBMC), Pogodinskaya Street, 10, build. 8, 119121, Moscow, Russia 2 Joint Institute for High Temperatures of Russian Academy of Sciences, Moscow, Russia 3 RES Ltd, Moscow, Russia 4 Foundation of Perspective Technologies and Novations, Moscow, Russia 5 Skryabin Moscow State Academy of Veterinary Medicine and Biotechnology, Moscow, Russia Correspondence should be addressed to Yu. D. Ivanov; yurii.ivanov.nata@gmail.com Received 27 March 2019; Revised 11 June 2019; Accepted 9 July 2019; Published 21 August 2019 Academic Editor: Carlos Michel Copyright © 2019 Yu. D. Ivanov et al. Tis is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Te concentration dependence of a microwave frequency radiation from a solution of a functioning enzyme system (ES) (with the example of cytochrome P450 BM3 (CYP102A1) system during lauric acid (LA) hydroxylation) has been studied with a radiothermometric sensor. Registration of the radiation from the enzyme solution has been performed in the frequency range from 3.4 to 4.2 GHz at the enzyme concentrations from 10 −10 V to 10 −6 V. It has been demonstrated that the catalysis of LA hydroxylation in a reconstituted CYP102A1 system is accompanied by a generation of microwave radiation over the entire range of concentrations studied. It has been found that a transition from a multipulse mode (at nanomolar enzyme concentrations from 10 −10 V to 10 −8 V) to a single-pulse mode (at micromolar enzyme concentrations from 10 −7 V to 10 −6 V) is observed. Tis efect is discussed on the basis of assumptions considering possible realization of biomaser-like radiation in the enzyme system. Te discovered concentration-based efect of the transition of an unsynchronized pulsed radiation into a synchronized one in ES can further be used in the development of novel methods of noninvasive diagnostics of diseases, in mathematical modeling of the functioning of living systems, and in the development of next-generation quantum computers. 1. Introduction Functioning of enzyme systems (ES) can be considered as a cyclic transition of the system from a nonexcited state to an excited nonequilibrium one and then back to the equilibrium. During the process of catalysis, a visible-range radiation can be observed for a number of enzyme systems, e.g., for the oxidative enzymes that catalyze the reaction followed by bioluminescence, i.e., luciferase [1]. Radiation from enzyme systems can be observed in other spectral ranges as well. In this way, previously it was demonstrated that the functioning of the CYP102A1 enzyme system in a narrow concentration range (from 10 −9 V to 10 −8 V) is accompanied by a microwave radiation in the form of pulse trains at 3.4 to 4.2GHz frequency [2]. In [2], a radiothermometry method was employed. Tis method is becoming more common for monitoring of functional state of both cellular systems and the whole organism [3, 4]. Radiothermometer (abbreviated, radiometer) measures the object’s brightness temperature   . Te brightness temperature (radio temperature) is the temperature value that is numerically equal to the thermo- dynamic temperature of an ideal black body. In the process of catalytic reaction, a nonequilibrium state of the medium can occur; this state is characterized by an increase in the brightness temperature, what can be accompanied by a radiation in a certain frequency range connected with the increase in the brightness temperature. Tus, the use of a radiometer operating in a microwave range allows one to measure the kinetics of biochemical processes by monitor- ing changes in the brightness temperature. Tis approach is very convenient, as it is label-free, does not require expensive equipment, and provides new information about Hindawi Journal of Sensors Volume 2019, Article ID 7608512, 11 pages https://doi.org/10.1155/2019/7608512