~ 278 ~ Journal of Entomology and Zoology Studies 2019; 7(5): 278-282 E-ISSN: 2320-7078 P-ISSN: 2349-6800 JEZS 2019; 7(5): 278-282 © 2019 JEZS Received: 13-07-2019 Accepted: 15-08-2019 Value Debbarma M.V.Sc. Scholar, Department of Animal Reproduction, Gynaecology and Obstetrics, Khanapara, Guwahati, Assam, India Sudip Sinha Professor, Department of Animal Reproduction, Gynaecology and Obstetrics, Khanapara, Guwahati, Assam, India L Kipjen Singh PhD Scholar, Division of Animal Reproduction, Gynaecology and Obstetrics, National Dairy Institute, Karnal, Haryana, Inida BC Deka Retd. Professor and Head, Department of Animal Reproduction, Gynaecology and Obstetrics, Khanapara, Guwahati, Assam, India RK Biswas Retd. Professor, Department of Animal Reproduction, Gynaecology and Obstetrics, Khanapara, Guwahati, Assam, India Rumi Saikia Borah Professor (Statistics), Department of Livestock Production Management, Khanapara, Guwahati, Assam, India T Gogoi Senior Scientist, Assam Agricultural University, Burnihat, Assam, India Dibakar Baruah Manager, District Poultry Farm, Demow, Sivasagar, Assam, India Correspondence Value Debbarma M.V.Sc. Scholar, Department of Animal Reproduction, Gynaecology and Obstetrics, Khanapara, Guwahati, Assam, India Effect of extenders on semen quality of Beetal bucks preserved at 5 °C Value Debbarma, Sudip Sinha, L Kipjen Singh, BC Deka, RK Biswas, Rumi Saikia Borah, T Gogoi and Dibakar Baruah Abstract A total of 22 pooled ejaculates comprising 94 ejaculates collected using artificial vagina from five adult Beetal bucks maintained at Goat Research Station, AAU, Burnihat were used to study the effect of extenders on the quality of buck semen preserved at 5 ºC. Each pooled ejaculate was split into three equal parts. The parts were extended using three extenders viz., Tris extender containing 20 percent egg yolk, Tris extender containing 1.5 percent soya lecithin and commercial Ovixcell extender by split-sample technique and then preserved in a refrigerator at 5 ºC. The extended semen samples were evaluated about two hours after collection before cooling to 5 °C and then at 24, 48, 72 and 96 hours of preservation at 5 °C for sperm motility, live sperm, intact acrosome and HOST-reacted sperm. The sperm motility and live sperm count in Tris extender containing 20 percent egg yolk were significantly ( P<0.05) higher than Tris extender containing 1.5 percent soya lecithin and Ovixcell extender. A significant ( P<0.05) decline was also observed in both sperm motility and live sperm count at each hour of preservation period in all the three extenders. The incidence of intact acrosome and HOST-reacted sperm in Tris extender containing 20 percent egg yolk was significantly (P<0.05) higher than the other two extenders and in Tris extender containing 1.5 percent soya lecithin than in Ovixcell extender. Both the acrosome and HOST-reacted sperm were significant (P<0.05) decline at each hour of the preservation period in all three extenders. It can be concluded that Tris-egg yolk extender is superior to Tris-soya lecithin and Ovixcell extenders for the preservation of buck semen at 5 °C. Keywords: Sperm, beetal bucks, HOST-reacted sperm, acrosome, Tris, Ovixcell 1. Introduction Goat is a multi-purpose animal that plays a significant role in the economy and nutrition of landless and marginal farmers in the country. Artificial insemination in goats is practiced in many countries, and although A.I. with frozen semen could reach acceptable fertility results of 55-65 percent (Salvador et al., 2007) [18] . The utilization of chilled liquid semen in a breeding program is an important alternative to A.I. with frozen semen which is more expensive. Moreover, fertility using semen stored at 5 °C is higher than that obtained with frozen semen (Paulenz et al., 2005) [15] . Freezing of semen has certain limitations which include decreases motility, interferes morphological integrity, increase embryonic loss and ultimately reduce fertility. Semen can be diluted and chilled as an alternative to freezing when insemination is performed within a short time after collection. However, the cool storage of semen from goat has received little attention during recent years. The media generally used for storage of chilled liquid buck semen (4-5 °C) are skim milk, sodium citrate-egg yolk and Tris-egg yolk diluents. Egg yolk lecithin and low-density lipoprotein has been reported to protect sperm membranes from cold shock and is a common constituent of the extenders used in the preservation of mammalian spermatozoa at low temperature (Leboeuf et al., 2000) [11] . The World Organization for Animal Health (OIE), recommended in the 2003 Terrestrial Animal Health Code, that animal origin product used in semen processing should be free of any biological risk (Marco-Jimenez et al., 2004) [12] . So, the search for non-animal origin, well-defined and contamination-free medium for extension of semen is highly desirable. Recently, several commercial vegetable origin extenders have been reported to show promising results that can be considered as extenders. Thus, the present study described the effect of the extenders on the quality of buck semen preserved at 5 ºC.