D. Rathi et al. International Journal of Recent Research Aspects ISSN: 2349-7688, Vol. 5, Issue 4, Dec 2018, pp. 1-5 © 2018 IJRRA All Rights Reserved page-1 Phytochemical Compound Analysis of Tinospora Cordifolia By GC-MS Method D.Rathi 1 , Dr.PL. Balasubramanian 2 1 Research Scholar, Department Of Siddha, Tamil University, Tamilnadu, India 2 Assistant. Professor, Department Of Siddha, Tamil University, Tamilnadu, India Abstract: The aim of the study was to carried out for identification of bioactive compounds from the whole plant methanol extract of Tinospora cordifolia by GCMS analysed by IIFPT (Iso/IEC 17025:2005). Referred standard protocol using NIST Library. The GCMS analysis revealed the presence of various compounds used for various disease. Key words: GC-MS, Tinopora cordifollia, Phytocomponents, I. INTRODUCTION The Tinospora cordifolia has been subjected to chemical investigation extensively and number of chemical constituents belonging to the different groups viz terpenoid, alkaloids, lignans, steroids have been analysed. Herbal plants produce and contain a variety of chemical substances with varied physiological effects. They are huge reservoir of various chemical substances with potential therapeutic properties [1]. Herbal plants are being increasingly utilized to treat a wide variety of clinical diseases [2]. Herbs have been used by all cultures throughout history and thus, herbal medicine is the oldest form of health care known to mankind. It was an integral part of the development of modern civilization. Many drugs commonly used today are of herbal origin. Higher plants as source of medicinal compound continue to play a dominant role in maintenance of human health since antiquities [3]. The primary benefit of using plant derived-medicine is that they are relatively safer than synthetic alternatives, offering profound therapeutic benefits and affordable treatment [4]. However, it must be noted that not all medicinal plants are safe for consumption in the crude form. Herbalism is a traditional medicinal or folk medicinal practice based on the use of plants and plant extracts. Herbal medicines are popular remedies for diseases used by a vast majority of the world‘s population,This study mainly focused on the bioactive compound ethonal extract Argemone mexicane plant seeds[4]. It is known as ‘Mexican prickly poppy’ and ‘Satyanashi’ is a common name. It is an erect, prickly annual herb, up to 1.2 meter in height, naturalized throughout India up to an altitude of 1,500 meter. Martials and method Extracted plant material powder by maceration method One liter of double distilled water was mixed with 100g of powdered Tinospora cordifolia stem, filtered twice with Whatman no.1 and then with nitrocellulose membrane. The extracted liquid was subjected to water bath vaporization to remove the water. For water bath evaporation, liquid extract material was be placed into a beaker and subjected to water bath evaporation at 60ºC temperature for 7-10 h daily for 2-3 days until a semisolid state of extracted liquid is obtained. The semisolid extract produced was kept in the deep freezer at-20ºC overnight and then subjected to freeze drying. Extract obtained by this method was then weighted and stored at 22ºC in desiccators until further use. The rat were fed with powdered plant material extract with sterile tap water. Phytochemical screening of the extract of Tinospora cordifolia was also carried out. The root of Tinospora cordifolia were first washed well and dust was removed from the plants. Plant was washed several times with distilled water to remove the traces of impurities from the root. The dried plant at room temperature and coarsely powdered. The powder was extracted with 70% ethanol for 48 hours. A semisolid extract was obtained after complete elimination of alcohol under reduced pressure. The extract was stored in refrigenerator used. The different concentrations (20mg/ml, 40mg/ml, 60 mg/ml and 80 mg/ml) respectively of plant extract used in this study. The stem, leaves and roots of Tinospora cordifolia were isolated, chopped into small pieces and dried roots were powered and this powder was used for the preparation of extract by heat distillation process as detailed by Agarwal et al.The extract was assigned a code name TCrE. II. ANALYSIS OF SAMPLES The given sample was extracted with methanol and analyzed through Gas Chromatography – Mass Spectrometry/ Mass Spectrometry for identification of different compounds (Indian Institute of Food Processing Technology, Ministry of Food Processing Industries, Government of India, Food Testing Laboratory [Nabl Accredited Laboratory As Per Iso/Iec 17025:2005] Fssai Referral Laboratory.