IOSR Journal of Biotechnology and Biochemistry (IOSR-JBB) ISSN: 2455-264X, Volume 5, Issue 1 (Jan. – Feb. 2019), PP 07-09 www.iosrjournals.org DOI: 10.9790/264X-0501010709 www.iosrjournals.org 7 | Page Role of high sensitive C–reactive protein in asthma patients Pallavi Mishra 1 , Dr. Prashant Hisalkar 2 , Dr. Neerja Mallick 3 , PhD Scholar, Dept of Biochemistry, People’s College of Medical Science & Research Centre, Bhopal, M.P. Professor & Head, Dept of Biochemistry, Govt Medical College, Dungarpur, Rajasthan, India Professor & Registrar, People’s University, Bhopal (M.P.) Corresponding Author: Dr Prashant Hisalkar Abstract: Asthma is a clinical condition characterized by inflammation of the airways and C-reactive protein is a marker for inflammation, infection, tissue damage and host defence mechanism by complement pathway. Impaired respiratory functions are associated with disturbance in immune response in the lung. This immune response is characterized by the secretion of some inflammatory cytokines such as C-reactive protein and IL-6. The case-control study was conducted in the department of Biochemistry, People’s College o f Medical Science & Research Centre Bhopal (M.P.). Total 200 subjects were selected for the study. The level of hs-CRP was measured using the nephelometric method. The mean value of serum high sensitive C-reactive protein was 4.74±2.24 in asthmatic cases and 2.53±1.31 in control group and p-value is ≤0.0001 which shows that serum hs-CRP was highly significant in asthmatic cases in comparison to the control group. It has been shown that serum level of hs-CRP in asthmatic patients were elevated compared with healthy controls and statistically highly significant difference was found. The overall finding shows that the High sensitive C-reactive protein is a marker for inflammation in asthma. Keywords: Asthma, Inflammation, hs-CRP, Immunity --------------------------------------------------------------------------------------------------------------------------------------- Date of Submission: 06-01-2019 Date of acceptance: 21-01-2019 --------------------------------------------------------------------------------------------------------------------------------------- I. Introduction Asthma is a clinical condition characterized by inflammation of the airways and C-reactive protein is a marker for inflammation, infection, tissue damage and host defence mechanism by complement pathway [1]. Infiltrations of the airway walls by mast cells, T-lymphocytes and eosinophils are involved in the inflammatory process [2, 3]. Impaired respiratory functions are associated with disturbance in immune response in the lung. This immune response is characterized by the secretion of some inflammatory cytokines such as C-reactive protein and IL-6 [4]. Assessment of serum hs-CRP levels has suggested the involvement of low-grade systemic inflammation in several disorders such as cardiovascular disease and diabetes mellitus [5, 6, 7, 8]. First it was separated by the serum of patient with acute inflammation. The C-reactive protein is precipitated by the somatic C-polysaccharide antigen of streptococcus pneumonia [9]. II. Materials And Methods The case-control study was conducted in the department of Biochemistry, People’s College of Medical Science & Research Centre Bhopal (M.P.). Total 200 subjects were selected for the study in which 100 asthmatic patients and 100 healthy subjects. The asthma diagnosis was established according to the Global Initiative for Asthma (GINA) guidelines. This study was approved by the institutional ethics committee and informed consent was obtained from all subjects. The inclusion criteria was patients having physician diagnosed asthma; patients who were not treated with any asthma therapy prior to the study; patients who were not on anti-histaminic, short and long-acting beta agonists; patients without any other lung disorder that could cause systemic inflammation other than asthma. The exclusion criteria had patients with upper or lower respiratory tract infection, trauma, collagen vascular disease, malignancy, smokers, alcoholics, pregnancy and lactation. The blood samples were collected and stored at -20˚C after serum separation. The level of hs-CRP was measured using the nephelometric method. Data were tabulated in Microsoft excel and perform analysis which is expressed as mean±SD. The statistical significance of differences between the means in the two groups was evaluated by using an unpaired T-test. p-value of <0.001 and p<0.05 considered highly significant and significant respectively.