_____________________________________________________________________________________________________ *Corresponding author: E-mail: pawoyesuku@yahoo.co.uk; Journal of Advances in Medicine and Medical Research 30(4): 1-9, 2019; Article no.JAMMR.50502 ISSN: 2456-8899 (Past name: British Journal of Medicine and Medical Research, Past ISSN: 2231-0614, NLM ID: 101570965) Knowledge, Uptake and Barriers to Pap Smear Test among Female Workers in the Rivers State University Teaching Hospital, Nigeria P. A. Awoyesuku 1* , B. O. A. Altraide 1 and D. A. MacPepple 1 1 Department of Obstetrics and Gynaecology, Rivers State University Teaching Hospital, 6-8 Harley Street, Old G.R.A, Port-Harcourt, Nigeria. Authors’ contributions This work was carried out in collaboration among all authors. Author PAA designed the study, performed the statistical analyses and wrote the first draft of the manuscript. Authors BOAA and DAM assisted in data collection, managed the analyses of the study and literature searches. All authors read and approved the final manuscript. Article Information DOI: 10.9734/JAMMR/2019/v30i430183 Editor(s): (1) Dr. Sevgul Donmez, Faculty of Health Sciences, Gaziantep University, Turkey. Reviewers: (1) Kiran Sharma, Mayo Clinic, USA. (2) Sivalingam Nalliah, International Medical University, Malaysia. Complete Peer review History: http://www.sdiarticle3.com/review-history/50502 Received 12 May 2019 Accepted 22 July 2019 Published 27 July 2019 ABSTRACT Background: Early screening for cervical cancer is a key intervention in reduction of maternal deaths. Health care workers have a significant role to improve cervical cancer screening practice among women. Their attitude and practice to such an issue might positively or negatively influence people they come into contact with. Objective: To determine the knowledge, uptake and barriers to Pap smear test among female workers in the Rivers State University Teaching Hospital. Methodology: A hospital-based cross-sectional study was conducted between September and November 2015. A structured and pre-tested questionnaire was used to collect data from 265 female hospital workers on socio-demographic characteristics, knowledge of Pap smear, attitude towards, as well as utilization of Pap smear test. The data obtained were analyzed using SPSS version 20.0. Results: Of the 265 respondents, 237 (89.4%) were aware of Pap smear while 28 (10.6%) had no knowledge; of those that had knowledge only 40 (16.9%) had Pap smear test done at least once Original Research Article