International journal of pharmaceutical science and health care Issue 2, Vol 6 December 2012 Available online on http://www.rspublication.com/ijphc/index.html ISSN 2249 – 5738 Page 116 Acute Sodium Selenite induced Physiological and Hematological alterations in Swiss Albino Mice N. Lakshmi Narasimha Murthy 1 , M. Vijaya Bhaskara Reddy 2 D. Adinarayana 1 , A. Bhuvaneswari 1 , S. Kishore 3 Corresponding author: Dr. M. Vijaya Bhaskara reddy Research Co-Ordinator Co-Principal Investigator Dept. of LPM College of veterinary Science Sri Venkateswara Veterinary University Tirupati-517502 Andhra Pradesh, India Cell: +91-7396120530 Authors: N. Lakshmi Narasimha Murthy, D. Adinarayana 1 , A. Bhuvaneswari 1 , S. Kishore 3 , Department of Zoology, Sri Venkateswar University 1,3 , Tirupati-517502, Andhra Pradesh, India, Cell: +91-9951201976 _____________________________________________________________________________________________________________________ ABSTRACT: The present study was aimed to assess the effects of sodium selenite on the neutrophil and the hematological parameters in mice. Thirty mice were randomly allocated into two groups fifteen animals in each group. Group 1 served as the control and 2 orally received sodium selenite orally at 1.5 mg Se/kg for 10 consecutive days. To assess the degrees of oxidative stress and of lipid peroxidation of the cellular membranes, the levels of thiobarbituric acid reactive substances (TBARS) were determined in serum samples that were collected on the 30 th day of experimentation. In addition, hematological parameters in the serum samples were measured by routine procedures. It was found that TBARS level in group 2 were significantly higher when compared to the basal level control group. These findings may explain the seemingly paradoxical effects of supplemental selenium on the indicators of oxidative stress, as the levels of TBARS were generally expected to decrease in the presence of selenium. Significant differences between the PCV in group 2 and RBC count in the group 2 was not significantly differed with control group. The white blood cell count (WBC) in group 2 showed a significant increase in