Pharmacophore, 10(4) 2019, Pages: 72-83 Pharmacophore ISSN-2229-5402 Journal home page: http://www.pharmacophorejournal.com Corresponding Author: Cesar G. Demayo; Department of Biological Sciences, College of Science and Mathematics, MSU- Iligan Institute of Technology, Iligan City 9200 Philippines. Email: cgdemayo@ gmail.com QUALITATIVE EVALUATION OF THE ANTIMICROBIAL, ANTIOXIDANT, AND MEDICINALLY IMPORTANT PHYTOCHEMICAL CONSTITUENTS OF THE ETHANOLIC EXTRACTS OF THE LEAVES OF GLIRICIDIA SEPIUM (JACQ.) WALP. Abdullah A. Abdulaziz 1 , Mark Lloyd G. Dapar 2 , Muhmin Michael E. Manting 1 , Mark Anthony J. Torres 1 , Agnes T. Aranas 3 , Roland Anthony R. Mindo 3 , Clint Kenny Cabrido 3 , Cesar G. Demayo 1 * 1. Department of Biological Sciences, College of Science and Mathematics, MSU-Iligan Institute of Technology, Iligan City, Philippines. 2. The Graduate School and Thomas Aquinas Research Complex, University of Santo Tomas, España, Manila, Philippines. 3. Chemistry Department, Ateneo de Davao University, Roxas Avenue, Davao City, Philippines. To Cite This Article: Abdullah A. Abdulaziz, Mark Lloyd G. Dapar, Muhmin Michael E. Manting, Mark Anthony J. Torres, Agnes T. Aranas, Roland Anthony R. Mindo and et al. (2019), “Qualitative Evaluation of the Antimicrobial, Antioxidant, and Medicinally Important Phytochemical Constituents of the Ethanolic extracts of the Leaves of Gliricidia sepium (Jacq.) Walp”, Pharmacophore, 10(4), 72-83. Introduction Medicinal properties of plants always have interested people since ancient times. Different cultures around the world have a variety of medicinal traditions that include the use of plants and other plant products in curing different diseases [1]. A report of the World Health Organization tells that 80% of the world population mainly rely on traditional therapies which involve the use of plant extracts or their active substances [2]. Plant products remain the principal source of pharmaceutical drugs and agents used in traditional medicine [3]. With about half a million plants around the world, medicinal plants have a promising future [4]. Medicinal plants are considered as a rich resource of ingredients which can be used in drug development including pharmacopoeial, non- pharmacopoeial, or synthetic drugs [5]. The modern pharmaceutical industry itself still relies largely on the diversity of secondary metabolites in plants and secondary metabolites, of which at least 12,000 have been isolated; a number estimated to be less than 10% of the total [4]. Through the Department of Science and Technology (DOST), the Philippine government is now addressing the need to assess scientifically the use of medicinal plants in the country [6]. ARTICLE INFO ABSTRACT Received: 04 th Apr 2019 Received in revised form: 12 th Aug 2019 Accepted: 15 th Aug 2019 Available online: 28 th Aug 2019 Keywords:Antimicrobial, Antioxidant, Phytochemical, Gliricidia sepium (Walp.) Jacq., GC-MS Analysis. The leaves of Gliricidia sepium (Jacq.) Walp. have been reported to have ethnomedicinal uses such as wound healing, skin itching or dermatitis, and others. This study was therefore conducted to evaluate its biological properties, whether ethanolic leaf extract of G. sepium (Jacq.) Walp. has antimicrobial and antioxidant activities, and whether these are reflected in the presence of antimicrobial and antioxidant phytochemicals in the extract. Antimicrobial properties using the agar well-diffusion method showed inhibition against bacterial organism Bacillus subtilis and fungus Candida albicans. The antioxidant activity evaluated by 2,2-diphenyl-1-picryl-hydrazyl-hydrate (DPPH) photometric assay showed that G. sepium (Jacq.) Walp. is a medium antioxidant with an IC50 of 144 ppm. Phytochemicals present in the extract showed the presence of flavonoids, saponins, steroids, and tannins, which are known as antimicrobial and antioxidant agents. Evaluation of the bioactive compounds in the extract using Gas Chromatography-Mass Spectrometry identified twenty-two (22) possible bioactive compounds. Of these, ten (10) were found to be known antimicrobials, while eight (8) compounds were found to be known antioxidants based on published literature. This study has shown support to the ethnomedicinal uses of the leaves of G. sepium (Jacq.) Walp. as an antimicrobial and antioxidant plant. Copyright © 2013 - All Rights Reserved - Pharmacophore