IOSR Journal of Dental and Medical Sciences (IOSR-JDMS) e-ISSN: 2279-0853, p-ISSN: 2279-0861.Volume 19, Issue 3 Ser.14 (March. 2020), PP 36-39 www.iosrjournals.org DOI: 10.9790/0853-1903143639 www.iosrjournal 36 | Page Effects of Ethanol Extract of Aloes (Aquilaria malaccesensis) Leaves in Lowering Blood Sugar Levels of Mice after Maltose Loading Adenin Dian Musrifani 1 , Yahwardiah Siregar 2 , M. Ichwan 3 123 Universitas Sumatera Utara, Medan, Indonesia Corresponding Author: Adenin Dian Musrifani Abstract Introduction: Diabetes mellitus is a significant health problem, in 2015 mentioned that around 415 million adults had a history of diabetes. Aloes leaves (Aquilaria malaccensis) contain flavonoid compounds which are phenolic compounds as antioxidants and are often used as antidiabetic. Efficacy of agarwood leaves as antidiabetic is related to secondary metabolite content from phytochemical screening, ethanol extract of fresh agarwood leaves and ethanol simplex extract obtained, there are active compounds such as flavonoids, glycosides, tannins, and steroids/triterpenoids. Objective: To determine the effect of agarwood leaf ethanol extract (Aquillaria malaccencis) in reducing blood sugar levels of mice after maltose loading. This study uses a randomized controlled design method with pre and post test control group design patterns. A total of 30 male mice (Mus musculus. L) aged 3-4 months weighing 20-35 grams were randomly selected and divided into 5 groups consisting of: Negative control group, positive control group, 2.5% EEDG (250 mg/kg/BW), 5% EEDG (500 mg/kg/BW), and 10% EEDG (1000 mg/kg/ BW). The extract was given first then after 10 minutes the whole group was burdened with maltose, measurements of blood sugar levels were carried out by measuring fasting KGD and KGD after loading of maltose at intervals of 30, 60 and 120 minutes. Methods: To see the differences between the control groups with the treatment carried out post-hoc test then performed LSD test, and calculate the value of the area under the curve (AUC) to see differences between groups. All data analyzes were performed using SPSS 17 software. In this study 95% confidence level was taken (p<0.05). Results: The study showed that the treatment group (EEDG dose 250 mg/kg/BW, EEDG dose 500 mg/kg/BW, and EEDG dose 1000 mg/kg/BW) had an effect in lowering blood sugar levels marked by significant differences with negative control group. EEDG dose of 500 mg/kg/BW obtained a significant value of p>0.05 ie p = 0.986 there was no significant difference with the positive control group. The under the curve (AUC) area shows the AUC value respectively produced by the negative control group (276.2 ± 8.980), the positive control group (217.9 ± 10.700), EEDG 250 mg/kg/BW (187.4 ± 33.862), EEDG 500 mg/kg/BW (217.6 ± 25.062), and EEDG 1000 mg/kg/BW (323.4 ± 40.482). Conclusion: From the entire treatment group the optimal dose was EEDG with a dose of 250 mg/kg/BW and it was able to significantly reduce the blood sugar levels of mice, but further research was needed for further active components of agarwood leaves that play a role in lowering blood sugar levels. Keywords: Aquillaria Malaccencis Leaf, Blood Sugar Level, Diabetes Mellitus, Maltose --------------------------------------------------------------------------------------------------------------------------------------- Date of Submission: 13-03-2020 Date of Acceptance: 28-03-2020 --------------------------------------------------------------------------------------------------------------------------------------- I. Introduction Diabetes mellitus (DM) is a collection of symptoms that arise in patients due to increased blood sugar (glucose) levels caused by insulin deficiency both in absolute and relative terms, so that regulation of blood sugar levels becomes chaotic. Although blood sugar levels are already high, the breakdown of fat and protein into glucose (gluconeogenesis) in the liver cannot be inhibited so that blood sugar levels increase (Soegondo, et al., 2005). Symptoms that often appear in people with DM are thirsty, easily hungry, frequent urination, and weight loss. Complications arising from DM include disruption of large blood vessels that can cause damage to the heart, brain and feet, as well as small blood vessels that can cause kidney, eye, and nerve damage (Utami, 2004). Α-glucosidase inhibitors work to inhibit the α-glucosidase enzyme in the small intestinal wall. The α- glucosidase enzymes (maltase, isomaltase, glucomaltase and sucrase) function to hydrolyze oligosaccharides and disaccharides in the small intestinal wall. Inhibition of the work of this enzyme can effectively reduce the