Research Article Total Phenolic, Flavonoid Contents, and Antioxidant Activities of Fruit, Seed, and Bark Extracts of Zanthoxylum armatum DC Nirmala Phuyal , 1,2 Pramod Kumar Jha, 1 Pankaj Prasad Raturi, 3 and Sangeeta Rajbhandary 1 1 Central Department of Botany, Tribhuvan University, Kirtipur, Kathmandu, Nepal 2 Forest Research and Training Centre, Ministry of Forests and Environment, Babarmahal, Kathmandu, Nepal 3 Ashok Medicinal and Aromatic Plants Center, Dabur Nepal Pvt. Ltd., Janagal, Kavre, Kathmandu, Nepal Correspondence should be addressed to Nirmala Phuyal; nirmalaphuyal@gmail.com Received 12 January 2020; Revised 31 January 2020; Accepted 17 February 2020; Published 16 March 2020 Academic Editor: Maria C. Yebra-Biurrun Copyright©2020NirmalaPhuyaletal.isisanopenaccessarticledistributedundertheCreativeCommonsAttributionLicense, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Natural antioxidants present in several medicinal plants are responsible for inhibiting the harmful effects of oxidative stress. ese plants contain polyphenols and flavonoids that act as free radical scavengers and reduce oxidative stress and may be an alternative remedytocurevariousharmfulhumandiseases.isstudyaimstoquantifythetotalphenolicandflavonoidcontents(TPCandTFC) andantioxidantpropertiesofmethanolextractsoffruits,seeds,andbarkofanimportantmedicinalandaromaticplant, Zanthoxylum armatum collectedfromwildandcultivatedpopulationsinNepal.TPCwasdeterminedbyFolin–Ciocalteucolorimetricmethodusing gallicacidasstandard,andvariousconcentrationsoftheextractsolutionsweremeasuredat760nm.TFCwascalculatedbyaluminum chloridecolorimetricassay.Quercetinwasusedasstandard,andtheabsorbancewasmeasuredat510nm.eantioxidantpotentialof thedifferentextractswasestimatedbyDPPHfreeradicalscavengingassay,andtheabsorbancewasmeasuredat517nm.ehighest TPC value was 226.3 ± 1.14mg GAE/g in wild fruits, and the lowest was 137.72 ± 4.21mg GAE/g in cultivated seeds. Similarly, the highest TFC value was 135.17 ± 2.02mg QE/g in cultivated fruits, and the lowest was 76.58 ± 4.18mg QE/g in cultivated seeds. e extracts showed variable antioxidant properties. e fruits exhibited excellent antioxidant properties with IC 50 values of 40.62 μg/mL and 45.62 μg/mLforcultivatedandwildfruits,respectively.Similarly,theIC 50 valuesofthebarkwere63.39 μg/mLand67.82 μg/mL, respectively, for cultivated and wild samples. And the least antioxidant capacity was shown by the seeds extract with IC 50 values of 86.75 μg/mLand94.49 μg/mLforwildandcultivatedseeds,respectively.eIC 50 valueofthestandardascorbicacidwas36.22 μg/mL. Differentextractsof Z. armatum containconsiderableamountofphenolsandflavonoids,includingantioxidantproperties,suggesting the potential use of this species in pharmacy and phytotherapy as a source of natural antioxidants. 1. Introduction Medicinal plants have been used in several indigenous herbalpracticessinceveryoldtimestocureseveraldiseases. Herbal medication still continues to serve as an important health care system even today despite the greater ad- vancements in modern medication systems in the recent years. eir long uses in the folk medicine and their safer implications in human health have generated much interest inthem,especiallyindevelopingcountries.Ithasnowbeen established that medicines derived from plant products are safer than their synthetic counterparts [1]. Plant and plant-based products are the natural sources of different phytochemicals such as phenols, flavonoids, alka- loids, glycosides, lignins, and tannins. Phenols and flavonoids are the most common phytoconstituents of different fruits, vegetables, and medicinal and aromatic plants, which are responsible for antioxidant activities [2]. Due to the potential toxicological effects of synthetic antioxidants [3], natural antioxidants such as phenols and flavonoid compounds from plant origin are gaining popularity these days [4]. An anti- oxidant is a substance that inhibit or delays oxidative damage to the cells of the organisms by scavenging the free radicals suchasperoxideorhydroperoxideandthusreducingtherisk Hindawi e Scientific World Journal Volume 2020, Article ID 8780704, 7 pages https://doi.org/10.1155/2020/8780704