World Journal of
Environmental Research
Volume 05, Issue 1, (2015) 118-121
www.awer-center/wjer/
Comparison of corncob and corn using as fungal culture medium
Jaruwan Chutrtong *, Industrial Microbiology Department, Suansunandha Rajabhat University, Bangkok 10300,
Thailand.
Suggested Citation:
Chutrtong, J. (2015). Comparison of corncob and corn using as fungal culture medium. World Journal of
Environmental Research. 5(1), 118-121.
Received February 08, 2015; revised March 23, 2015; accepted April 05, 2015
Selection and peer review under responsibility of Dr. Nehir Varol, Near East University, Cyprus.
©2015 SPROC LTD. Academic World Education & Research Center. All rights reserved.
Abstract
Corn or maize is the most widely grown grain crop. It is very practical. It is used as staple food, major source of
cooking oil and grown to feed for livestock. Starch from maize can be made into syrups plastics, fabrics,
adhesives, and many other chemical products and also fermented and distilled to produce alcohol. This research
aims to compare pigment synthetic of Neurospora sp., widely used as a model organism in genetics research,
when use corn, dry corncob and potato dextrose agar as culture medium. After fully grow up, extracted
mycelium pigment with 95% ethyl alcohol. Then measure the absorbance of the pigment by spectrophotometer
at wavelength 450.5 nanometer. The results showed that the best growth of Neurospora cultured on dry
corncob was sample which added water (for moisture) 90 % w/w. While the best growth of Neurospora cultured
on corn was sample which added water only 15 % w/w. When compare the intensity of mycelium pigment, it
was found that corncob is the best medium for pigment synthetic of Neurospora. Absorbance of pigment
extract from mycelium culture on corncob was 0.64(diluted 5X). As the absorbance of pigment extract from
mycelium culture on corn and PDA were only 0.34(diluted 5X) and 0.35(diluted 2X).
Keywords: corn, corncob, fungal, medium.
*ADDRESS FOR CORRESPONDENCE: Jaruwan Chutrtong, Industrial Microbiology Department, Suansunandha Rajabhat
University, Bangkok 10300, Thailand. E-mail address: jaruwan.ch@ssru.ac.th