MORPHOLOGICAL AND BIO Sowmya Poosapati, *Rav ICAR-Indian Institute o ARTICLE INFO ABST The st stress T. asp charact compa micros reduce of redu of vacu isolates isolate thus it Copyright©2016, Sowmya Poosapati et al. This is an op use, distribution, and reproduction in any medium, provi INTRODUCTION Trichoderma species are the most biopesticides in today’s agriculture (Verm Trichoderma antagonizes phytopathogenic antifungal properties are principally due t produce antibiotics (Vinale et al., 2008) enzymes (Benitez et al., 2004) and competit nutrients (Elad, 2000). Even through Tric promote plant growth and induce resistan abiotic stresses (Hermosa et al., 2012), t pesticide has primarily contributed to their co as bio-agents. Nevertheless, Trichoderma b not immune to abiotic stress like moisture temperature, etc., that tend to cause physiological, biochemical and molecul adversely affect the beneficial consequ bioagents. For example, soil hydrological co the growth and antagonistic properties (Tronsmo and Dennis, 1978; Luard and Grif 1988) and soil temperature affect the ra Trichoderma (Knudsen and Bin, 1990). *Corresponding author: Ravulapalli Durga Pras ICAR-Indian Institute of Oilseeds Researc Hyderabad – 500030 ISSN: 0975-833X V Article History: Received 22 nd June, 2016 Received in revised form 29 th July, 2016 Accepted 27 th August, 2016 Published online 30 th September, 2016 Key words: Thermotolerance, Trichoderma, Conidial morphology, Lytic enzymes, SEM, TEM. Citation: Sowmya Poosapati, Ravulapalli Durga thermotolerant Trichoderma”, International Journal o RESEARCH ARTICLE OCHEMICAL CHARACTERIZATION OF TH TRICHODERMA vulapalli Durga Prasad and Navaneetha of Oilseeds Research, Rajendranagar, Hyderaba TRACT tudy focuses on the morphological and physiological ce in thermotolerant isolates of Trichoderma viz., T. long perellum 7316 (TaDOR7316). Heat shock of 52 °C le teristic viz., decrease in the size and volume of spores but th arison to susceptible Trichoderma isolates tested. Sca scopy (TEM) of fungal cultures heated at 52 °C demonst ed intracellular spaces and mitochondrial count. The observa uced metabolism during stress conditions. TEM analysis als uoles indicating their role as scavengers of toxic metabolite s showed different patterns of lytic enzyme production and w T. asperellum TaDOR7316 was able to produce β-1, 4-gl can be further tested for biological control of plant pathogen pen access article distributed under the Creative Commons Attribut ided the original work is properly cited. commonly used ma et al., 2007). c fungi and the to their ability to and/or hydrolytic tion for space and choderma species nce to biotic and their role as bio- ommercial success by themselves are deficiency, higher e morphological, lar changes and uences of these onditions influence of Trichoderma ffin, 1981; Magan, adial extension of sad, ch, Rajendranagar, Thermotolerant strains have agents Bacillus, Pseudomona Trichoderma (Poosapati et al., thermotolerant isolates of Trich (Poosapati et al., 2014; Prasad study these isolates were used physiological changes associa The preliminary observations for understanding the mechanis these in turn can be employed f biocontrol agents. MATERIALS AND METH Strains The thermotolerant strain T. longibrachiatum 673 (TaDO (TaDOR7316), which were ide in Microbial type culture c Chandigarh, India (Poosapati e study. These thermotolerant str of Trichoderma isolates, isolat from various regions of India. T locus sequencing (elongatio polymerase subunit B) was Available online at http://www.journalcra.com International Journal of Current Research Vol. 8, Issue, 09, pp.38668-38672, September, 2016 I Prasad and Navaneetha Tippirishetty, 2016. “Morphological of Current Research, 8, (09), 38668-38672. HERMOTOLERANT a Tippirishetty ad – 500030 ell responses to high temperature girachiatum 673 (TaDOR673) and ed to changes in morphological he reduction was significantly less in anning and transmission electron trated shrinkage of cytoplasm and ations have supported the hypothesis so revealed increased accumulation es generated during heat stress. The we observed that the thermotolerant lucanases even after heat stress and ns under stress conditions. tion License, which permits unrestricted been identified in bio-control as (Kumar et al., 2014) and 2014). The biocontrol ability of hoderma were established earlier d et al., 2016) and in the current to study the morphological and ated with heat stress tolerance. provide supportive information sms of heat stress tolerance and for the improvement of potential HODS ns of Trichoderma viz., OR673) and T. asperellum 7316 entified previously and deposited collection (MTCC), IMTECH, et al., 2014), were used for this rains were identified from a pool ted from soil samples collected To confirm their identity, multi- on factor1 alpha and RNA performed using the primer INTERNATIONAL JOURNAL OF CURRENT RESEARCH and biochemical characterization of