UniversePG I www.universepg.com Antibacterial Activity of Cissus quadrangularis Stem Extract on the Pathogenic and Industrial Waste Watered Bacteria Md. Golam Mosaib 1 , Md. Abdullah Al Maruf 2 , Rabiul Islam 3 , Shahriar Mahmud 4 , Shaharuq Nahid Sohana 4 , Md. Abu Sayeed Imran 4 , Mehadi Hasan Rony 4 , Maidul Islam 5 , Fatema Tuz Zuhora 6 , and Shafiqul Islam 6 1 Dept. of Biochemistry and Molecular Biology, Gono Bishwabidyalay, Savar, Dhaka, Bangladesh; 2 Dept. of Biochemistry and Molecular Biology, Jahangirnagar University, Dhaka, Bangladesh; 3 Divisional DNA Screening Laboratory, Faridpur Medical College Hospital, Ministry of Women & Children Affairs, Faridpur, Bangladesh; 4 Dept. of Biotechnology and Genetic Engineering, Islamic University, Bangladesh; 5 Apex Biotechnology Laboratory, Dhaka, Bangladesh; and 6 Dept. of Microbiology, Gono Bishwabidyalay, Savar, Dhaka, Bangladesh *Correspondence: gmosaib@gmail.com ABSTRACT Cissus quadrangularis (Vitaceae) is a popular climber conspicuous by its flesh quadrangular stem widespread throughout the Bangladesh. The in vitro antimicrobial activity of C. quadrangularis extracts was studied against selected pathogenic bacteria, industrial wasted bacteria, and broth dilution assay. The most commonly used method of microbiological assay is the disc diffusion method. C. quadrangularis stem extracted with four solvents (Petroleum spirit, methanol, ethyl acetate, and dichloromethane) were tested for antimicrobial activities against some pathogenic microorganisms Sarcina lutea (002-1), Xanthomonas campestris (004-1), Escherichia coli (005-1), Klebsiella pneumonia (006-1) and some industrial (Tannery, Tobacco, and Sugar mill) waste watered bacteria by disc diffusion method. Among the four extracts, ethyl acetate showed moderate antibacterial activity against X. campestris (004-1) and industrial watered bacteria. But, the commercial disc Oxicycline doesn’t show any antibacterial activity against the industrial waste watered bacteria. Petroleum spirit, methanol, and dichloromethane extract were ineffective against all of the tested bacteria. Keywords: Cissus quadrangularis, Antibacterial effect, Industrial waste, Stem extract, Pathogenic, and Tannery. INTRODUCTION Medicinal Plants have been used as a source of medicine since the dawn of civilization. The plant designed as medicinal is implied that it is useful as a drug or therapeutic agent or an active ingredient of a medicinal preparation. Various medicinal plants have been applied for years in daily life to treat disease all over the world (Nair et al., 2004). The stem contains two unsymmetrical tetracyclic triterpenoids, and two steroidal principles. The presence of β-sitosterol, δ- amyrin, δ-amyrone, and flavonoids (quercetin) having different potential metabolic and physiological effects have also been reported (Jainu and Devi, 2004). The ulcer protective effect of a methanolic extract of C. quadrangularis was similar to that of the reference medicine sucralfate (Jainu and Devi, 2004). Many imitate agents such as estrogens in hormone replacement therapy, especially estrogen receptor modulators have been designed to treat osteoporosis but each one of them is linked with side effects such European Journal of Medical and Health Sciences, 2(2), 28–38, 2020 Publisher homepage: www.universepg.com, ISSN: 2663-7529 (Online) & 2663-7510 (Print) https://doi.org/10.34104/ejmhs.020.28038 European Journal of Medical and Health Sciences Journal homepage: www.universepg.com/journal/ejmhs 28