Int.J.Curr.Microbiol.App.Sci (2019) 8(6): 1821-1826 1821 Original Research Article https://doi.org/10.20546/ijcmas.2019.806.217 In-vitro Efficacy of Botanical and Selected Bio-Agents in the Management of Fusarial wilts of Tomato (Lycopersicon esculentum L.) Vinny John*, Sunil Zacharia, Amit Kumar Maurya, Anoop Kumar and Sobita Simon Department of Plant Pathology, Sam Higginbottom University of Agriculture Technology and Sciences, Prayagraj, India *Corresponding author ABSTRACT Introduction Tomato is a most popular widely grown vegetable in the world. Both the species and its use as a food originated in Mexico, and spread around the world (Nem Pal Singh et.al 2004). Edible fruit, often red in color from the plant (Lycopersicon esculentum) from a member of family solanaceae is mainly cultivated as vegetable. It is a rich source of vitamin C; it also contains minerals like calcium, phosphorus (Linger and Hill 1991). As it is a short duration crop and gives high yield, it is important from economic Point of view and hence are under its cultivation is increasing day by day. Fusarium oxysporum f. sp. lycopersici (FOL) is a highly destructive pathogen of both green house and field grown tomatoes in worm vegetable production areas. The disease caused by this fungus is International Journal of Current Microbiology and Applied Sciences ISSN: 2319-7706 Volume 8 Number 06 (2019) Journal homepage: http://www.ijcmas.com The study was conducted in the department of Plant Pathology SHUATS Allahabad (2014) to investigate mycoparasitism inhibitory effects of Trichoderma spp, Pseudomonas sp, neem leaves extract and carbendazim on the growth of the Fusarium oxysporum f.sp. lycopersici which causes wilt of tomato in-vitro, Trichoderma harzianum, Trichoderma viride, Pseudomonas fluoresens were used @ 2% (dual culture technique) and carbendazim @0.1gm and Neem leaves extract in the ratio of 1:1(poison technique). Among all Trichoderma viride was the best inhibitor of test pathogen (87.44%) followed by carbendazim (87.11%). Result showed that all the treatments significantly inhibit the growth of the pathogen (%). Keywords Carbendazim, Fusarium oxysporum f. sp. lycopersici, Lycopersicon esculentum, Neem leaf extract, Pseudomonas fluorescens, Trichoderma harzianum, T. viride Accepted: 15 May 2019 Available Online: 10 June 2019 Article Info