INDUCED ASH TOXICITY EFFECTS ON THE HEMATOLOGICAL AND BIOCHEMICAL CHANGES OF CYPRINUS CARPIO L. 1785 Noor A. Sh. Al-Naymi 1 , Muhanned R. Nashaat 2* , Ahmed J. Mohammed 3 1 Directorate of 1 st Karkh/ Ministry of Education, Baghdad, Iraq. 2* Ministry of Science & Technology, Baghdad, Iraq. 3 Department of Biology, College of Science, University of Baghdad, Baghdad, Iraq. *e-mail : muhanned_nashaat@yahoo.com (Received 28 November 2018, Revised 27 March 2019, Accepted 31 March 2019) ABSTRACT: This study was the first of its kind in Iraq, which aims to assess the risk of Durah power plant ash concentrations in hematological and biochemical changes of common carp. The result shows that the mean RBC value was from 1.3±0.015 to 0.93±1.54 was recorded in a group of 0.5and3ppt after two-six weeks of exposure, respectively. In contrast, the mean Hb value were from 10+0.26 to 6+0.11g/dl was recorded in blood sample exposed to 0.5and3ppt after two-six weeks of exposure, respectively. Regarding to PCV was decreased from 30%±0.54 to 18%±0.74 at the end of 45 th days of exposure in treated group as compared to control group. Moving to WBCs increased from 10.87±0.31mm 3 in 0.5ppt to 20.4±0.14mm 3 at the 3ppt concentration after six weeks of exposure period. Turning now to activity of liver enzymes the results of SGPT value were from72±1.665 to126±0.48U/L was recorded in blood sample exposed to 0.5and3ppt after two-six weeks, respectively. All these data were higher than control group. However the mean SGOT values were from 274±13.52 to397±18.42 U/L was recorded in blood fish exposed to 0.5and3ppt after two-six weeks, respectively.Our study concluded that presence of toxic ash in aquatic environment may produce systemic toxic effects and reveal the changes in blood cells count of fish also pose hazardous alternations in biochemical parameters. Key words : Fly ash, Durah Power Plant, common carp, hematological, SGOT, SGPT. Biochem. Cell. Arch. Vol. 19, No. 2, pp. 2983-2989, 2019 www.connectjournals.com/bca ISSN 0972-5075 INTRODUCTION Blood parameters are considered as the pathophysiological indicators of the whole body and are important in diagnosing the structural and functional process in fish. Hematological studies are important to know the impact of pollutions on aquatic organisms which along with indicating the physiological disturbances also provides vital information on general fish health (Barton and Iwama, 1991). In recent years the energy demands have been increased so rapidly which is beanies largely met by using fossil fuel, this increasing is considered one of the challenges that faces the development of the country (Bashar, 2010). The main source of energy is thermal power plants were produced the energy by using the coal as the fossil fuel. The process of coal combustion caused in health and environmental effects. Especially, coal is containing a largely mounts of organic and inorganic compound such as heavy metals, Poly Aromatic Hydrocarbons (PAHs) and Poly Chlorinated Biphenyls (PCBs) which are transformed to surrounding environment by various pathways. Heavy metals pollution has been indicated in aquatic animals (Adham et al, 2002; Olojo et al, 2005). This contamination’s impact on the potential toxicity for the humans and their environment (Lee et al, 2007; Gueu et al, 2007). Fly ash is a waste material resulting from coal based on thermal power plant which continuously increasing source of pollution chemically ash components consist a large amount of heavy metals, cations, anions, PAHs, PCBs, and quantity of various potentially toxic metals. Fish are located at the end level of the aquatic food chain and can’t escape from the impact of these pollutants, which may bioaccumulation and pass through the food to humans with causing acute and chronic diseases (Farombi et al, 2007). Therefore, the current study efforts have been made to indicate the toxic effect of fly ash contamination on hematological and biochemical parameters of C. carpio as a test organism under the control and treated conditions DOI : 10.35124/bca.2019.19.2.2983