www.ejbps.com Sahu et al. European Journal of Biomedical and Pharmaceutical Sciences 208 QUALITATIVE PHYTOCHEMICAL SCREENING OF LEAVES AND STEM OF IPOMOEA CARNEA Kaminee Sahu 1 * and Dr. Seema Kohli 2 1 Research Scholar, Bhagwant University, Ajmer , Rajesthan. India. 2 Department of Pharmacy, Kalaniketan Polytechnic Collage Jabalpur, M.P. India. Article Received on 26/08/2016 Article Revised on 15/09/2016 Article Accepted on 06/10/2016 INTRODUCTION Common name of Ipomoea carnea is Bush Morning Glory, Morning Glory Tree, Besharam, Behaya and shameless. It is belongs to the family Convolvulaceae. [1] the plant well distributed in India and found particularly in Chhattisgarh and Madhya Pradesh. Preliminary phytochemical analysis show the presence of possible secondary metabolites, namely alkaloids flavonoids, terpenoids, cynogenic glycosides, phenolics, tannins and saponins. [2][3] The flowers contain the maximum and the stem contains the minimum amount of phenols. The flavonoid content of the flowers was quite high compared to that of the leaves and the stem. [4] Other uses of Ipomoea carnea are Ipomoea carnea stem can be used for making paper. Stem is also used as fire wood. Leaves are used as fertilizer. The plants are also used for fencing. Colorful flowers of plant are often grown as ornamentals. The plant has various medicinal value. It has sedative and anticonvulsant properties. A glycosidic saponin of Ipomoea carnea has anticarcinogenic and oxytoxic properties. [5] COLLECTION, AUTHENTIFICATION AND EXTRACTION OF PLANT MATERIAL The whole selected plant was collected from road side fencing shrub of Ipomoea carnea (Fam. Convolvulaceae). Fresh leaves and stem of selected plant was collected in the month of december from Jabalpur Madhya Pradesh, India. The leaves and stem was dried under shade then stored in air tight container for further studies. The plant was authenticated by the Taxonomist, Dr. A. B. Tiwari, Associate Professor, Department of Plant Physiology, Jawaharlal Nehru Krishi Vishwa Vidyalaya, Jabalpur, Madhya Pradesh, India. Preparation of various solvent extract The leaves and stems were washed under running tap water to remove the surface pollutants. Fresh leaves and stems of Ipomoea carnea were shade dried and powdered (by using pastle motor) then extracted with solvent system ethanol, aqueous medium and mixture of solvents, water. [6] The plant possess various bioactive compounds such as glycosides, alkaloids, reducing sugars, flavonoids, fatty acid, esters, alcohol and tannins. The leaves of this plant showed the presence of thirteen compounds which include hexadecanoic acid, stearic acid, 1, 2 diethyl phthalate, n-octadecanol, octacosane, hexatriacontane, tetracontane, 3- diethylamino-1- propanol. [7][8] SJIF Impact Factor 3.881 Research Article ejbps, 2016, Volume 3, Issue 11, 208-211. European Journal of Biomedical AND Pharmaceutical sciences http://www.ejbps.com ISSN 2349-8870 Volume: 3 Issue: 11 208-211 Year: 2016 * Corresponding Author: Kaminee Sahu Research Scholar, Bhagwant University, Ajmer , Rajesthan. India. Mail ID: kaminisahu26@gmail.com ABSTRACT The undertaken study aims at the screening of leaves and stems of Ipomoea carnea for various active constituents. Leaves and stems of the plants were extracted with water and ethanol and the extract obtained was tested for the presence for chemical constituents. The result showed that the presence of alkaloids, carbohydrates, steriods and sterol, glycosides, saponins, flavonoids, phenolic compounds, triterpenoids in ethanolic and aqueous extracts of leaves and stem. The phytochemical screening for leaves and stem of ipomoea carnea has played an important role in the pharmacological activity of the plant. The results have shown that ethanolic and aqueous extract of the leaves and flower of Ipomoea carnea has higher concentration of flavonoids (which is responsible for pharmacological activity) compared to phenolic compounds. KEYWORD: glycosides, saponins, flavonoids, phenolic compounds, triterpenoids.