Original Research Article http://doi.org/10.18231/j.ijpca.2019.022 International Journal of Pharmaceutical Chemistry and Analysis, October-December, 2019;6(4):120-126 120 Analytical studies on zero order and first order derivative and area under curve UV-spectrophotometric methods for estimation of pimavanserin tartrate in bulk and In-house tablet formulation Abdul Talib Abdul Wahab 1 , Mohammad Mojeeb Gulzar Khan 2* , Atul Arun Shirkhedkar 3 1 Research Scholar, 2 Associate Professor, 3 Professor, Dept. of Pharmaceutical Chemistry, 1-3 R.C. Patel Institute of Pharmaceutical Education and Research, Shirpur, Dhule, Maharashtra, India *Corresponding Author: Mohammad Mojeeb Gulzar Khan Email: mujeebgulzar@gmail.com Abstract The proposed of work to establish simple, rapid, sensitive, economical and accurate UV spectrophotometric methods for the quantificationof Pimavanserin tartrate in bulk material and in-house tablet formulation. This study is designed to validate the developed methods as per ICH guidelines. Pimavanserin tartrate an atypical anti-psychotic drug also used for the treatment of Parkinson Disease. Four simple UV Spectroscopy methods developed and validated for the estimation of Pimavanserin tartrate, by using double beam spectrophotometer (UV-2450, Shimadzu, Japan). Maximum absorbance (λmax) of pimavanserin tartrate was observed at 226 nm used methanol as a solvent. The calibration curve of concentration range 5-30 μg/ml obeyed Beer lambert law. The % recovery was found to be in the range of 98-101%. Precision values observed less than 2 in the terms of % RSD that shows precise nature of developed methods. It was concluded that statistical analysis and the result amongst all four methods, AUC method is most simple, specific, accurate and precise. All four methods can be used as routine analysis of Pimavanserin tartrate in bulk and pharmaceutical formulations. Keywords: Area under curve, Derivative-spectrophotometry, Pimavanserin tartrate, UV Spectroscopy. Introduction Pimavanserin tartrate (PMT) is chemically (2R, 3R) - 2,3 - dihydroxybutanedioic acid; 1-[(4-fluorophenyl) methyl] -1- (1-methylpiperidin-4-yl) -3- [[4-(2- methylpropoxy) phenyl] methyl]urea (Fig. 1). Pimavanserin tartrate (PMT) is one of the atypical anti-psychotic which is also used for the treatment of Parkinson. 1 When it synthesized Pimavanserin has been administered as its tartrate salt. 2 PMT is not a dopamine receptor antagonistbutis having inverse agonist on 5-HT2A 3-7 subtype receptor for the treatment of psychosis in Parkinson‟s disease (PD) and used in the treatment of schizophrenia. It‟s also having significant effect on insomnia, it‟s selective serotonin 5- HT2A receptor inverse agonist, to the slow wave sleep. 8 Literature survey gives details about analytical methods for determination of PMT. In High Performance Liquid Chromatographic used to Quantification of Pimavanserin in Bulk and Tablet Dosage Form Using A Stability Indicating method, 9 ultrafast LC for estimation pimavanserin in pharmaceuticals. 10 and UPLC-MS for estimation of Pimavanserin tartrate in rat plasma 11 but still no UV spectrophotometry method has been developed for the determination of PMT in bulk and tablet formulation. At the same time, our goal was use to AUC and amplitude technique to established zero order and first order derivative. Fig. 1: Chemical structure of pimavanserin tartrate