ORIGINAL ARTICLE Discovery Phytomedicine 2018, Volume 5, Number 4: 48-51 48 Discovery Phytomedicine 2018; 5(4): 48-51. doi: 10.15562/phytomedicine.2018.70 www.phytomedicine.ejournals.ca CrossMark ABSTRACT Hevea brasiliensis, a plant belonging family Euphorbiaceae. In Brazil this plant is not only use for medicinal purpose but also for cosmetics purpose. The present study was aimed to study analgesic and antidiarrheal activity of methanol extract of Hevea brasiliensis. Analgesic activity was evaluated by acetic acid- induced writhing method and antidiarrheal by gastrointestinal motility method (charcoal meal test) in mice. Phytochemical evaluation was carried out by qualitative analysis. For analgesic evaluation, the extract (250 mg and 500 mg) showed significant activity compared to control diclofenac Na. On the other hand, for antidiarrheal activity the extract (250 mg and 500 mg) significantly reduce charcoal propulsion. The phytochemical evaluation showed significant presence of Alkaloids, Carbohydrates, Glycosides, Saponins, Phytosterols, Proteins and amino acids, Fats & fixed oils. It’s concluded that the extract possesses both analgesic and antidiarrheal activity and containing wide range of phytochemicals. Keywords: Analgesic, Phytochemicals, Antidiarrheal, Hevea brasiliensis INTRODUCTION Hevea brasiliensis (H. brasiliensis), better known as the rubber tree, is the primary source of natu- ral rubber. Hevea brasiliensis, a plant belonging to the family Euphorbiaceae, is widely used for the production of rubber. Several rubber producing countries are expanding cultivation of rubber to areas outside the scope of optimum weather conditions in order to meet increasing demand for natural rubber, capture otherwise ‘waste’ land, enhance economic activities of rural dwellers and boost of export trade to earn foreign exchange. In addition, H. brasiliensis is cultivated in non-traditional areas as escape zone for some devastating diseases such as the South American Leaf Blight in Brazil. 1 Rubber tree is a quick-growing tree which is the most economically important member of the genus Hevea. It is of major economic importance because the milky latex extracted from the tree is the primary source of natural rubber. As a latex producing crop, the bark is regularly tapped. 2,3 Latex, the source of hevea or para rubber, is obtained by tapping the trunks of the trees. It is cultivated for rubber, food, apiculture, fibre, timber, lipid, fuel etc. It also cultivated for soap, insect repellent in Brazil. The extract from H. brasiliensis possesses antimicrobial activities against E. coli, Klebsila pneumoniae and Pseudomonas aeruginosa. 3,4 Proteins present in natural rubber latex may cause allergic reactions. 5 In this study, we aimed to evaluate the analgesic and antidiarrheal activity of methanol extract of Hevea brasiliensis. MATERIALS AND METHODS Plant collection and identification Te roots of Hevea brasiliensis was collected from Fatikchari, Chittagong, July, 2013. It was taxo- nomically identifed by the experts of Bangladesh National Herbarium, Mirpur, Dhaka, Bangladesh (Accession number-38637) and also deposited there for further study. Preparation of Hevea brasiliensis extract Afer collection, the roots were cut into pieces, dried under sun for three weeks and fnally ground to coarse powder by a suitable grinder. According to 1 Department of Pharmacy, Noakhali Science and Technology University, Bangladesh 2 Department of Pharmacy, University of Dhaka, Bangladesh 3 Department of Microbiology, Noakhali Science and Technology University, Bangladesh 4 Department of Pharmacy, East West University, Dhaka, Bangladesh 5 Department of Pharmacy, Atish Dipankar University of Science and Technology, Bangladesh 6 Department of Pharmaceutical Sciences, North South University, Bangladesh * Corresponding Author: Md Lukman Hakim, Department of Pharmaceutical Sciences, North South University, Bangladesh amin.pharma07@gmail.com Cite This Article: Seeam, S.M., Islam, A.S.M.M., Raju, G.S., Istiaq, A., Rana, M.D.S., Babu, M.H., Rahman, M.D.M., Islam, M.D.J., Hakim, M.D.L., Das, P. 2018. Biomedical and Phytochemical Study of Methanol Extract of Hevea Brasiliensis Roots in Swiss Albino Mice. Discovery Phytomedicine 5(4): 48-51. DOI:10.15562/ phytomedicine.2018.70 Biomedical and Phytochemical Study of Methanol Extract of Hevea Brasiliensis Roots in Swiss Albino Mice Saifuddin Mohammad Seeam, 1 A S M Monirul Islam, 2 Golam Sarwar Raju, 1 Arif Istiaq, 3 Md. Sohel Rana, 4 Mohammad Hasem Babu, 5 Md. Masudur Rahman, 5 Md. Jahirul Islam, 5 Md Lukman Hakim, 6* Palash Das 5