IP International Journal of Medical Microbiology and Tropical Diseases 2019;5(4):188–192 Content available at: iponlinejournal.com IP International Journal of Medical Microbiology and Tropical Diseases Journal homepage: www.innovativepublication.com Original Research Article Antimicrobial susceptibility profile of Staphylococcus aureus isolated from pyogenic infections-Variations encountered at secondary and tertiary care level centres Mandeep Sen 1 , Sana Islahi 1, *, Anupam Das 1 , Jyotsna Agarwal 1 1 Dept. of Microbiology, Dr. Ram Manohar Lohia Institute of Medical Sciences, Lucknow, Uttar Pradesh, India ARTICLE INFO Article history: Received 24-10-2019 Accepted 15-11-2019 Available online 11-01-2020 Keywords: Pyogenic Infections Secondary and Tertiary Health Care Level Centre Antimicrobial Resistance ABSTRACT Introduction: The bacterial profile of pus samples in many studies remain the same, but the antibiotic resistance pattern of these isolates has shown a lot of variations. Objective: Comparative study of bacteriological profile and antimicrobial susceptibility of Staphylococcus aureus isolated from pus and wound swab of patients at secondary and tertiary care level centres. Material and Methods: This observational retrospective study was conducted at the department of Microbiology in a super specialty Post Graduate institute. Antibiotic sensitivity testing of Staphylococcal isolates was performed by modified Kirby Bauer’s disc diffusion method as per CLSI 2017 and EUCAST guidelines. Automated susceptibility testing was performed by Vitek-2 Compact system. Conclusions: This study provides a comparative All medical pus specimens from both institution as well as from SRCLI (State Referral Centre for Clinical Lab Investigation) were included of Staphylococcus aureus isolated from pus and wound swab of patients to formulate the local antibiotic policy to initiate the appropriate empirical antibiotic treatment at secondary and tertiary care level centres. Results: Out of 997(59.38%) samples showing single growth, Gram positive organisms were 216 (35.23%) for institutional and 195 (50.78%) for SRCLI (State Referral Centre for Clinical Lab Investigation). 85% and 91% isolates of Staphylococcus aureus were resistant to penicillin followed by erythromyc in (53.5% and 49%) and cefoperazone-sulbactum (28.5% and 14%), while 31 (55%) and 36 (34.61%) isolates were MRSA respectively for institutional and SRCLI. Even the higher antibiotic like linezolid showed 2.5% resistance (P = 0.5054) in MRSA isolates of SRCLI compared to 0% in institutional samples, which was, though not statistically significant, but is of great concern. Vancomycin showed no resistance in both areas. © 2019 Published by Innovative Publication. This is an open access article under the CC BY-NC-ND license (https://creativecommons.org/licenses/by/4.0/) 1. Introduction Wound infection can be caused by variety of organ- isms which may co-exist as polymicrobial communities. Although, the bacterial profile of pus samples in many studies remain the same, the antibiotic resistance pattern of these isolates has shown a lot of variations. Staphylococcus aureus is a Gram positive cocci, which is a part of normal flora occupies anterior nares, nasopharynx, perineal area, skin and colonizer of mucosa. S.aureus is one of the emerging pathogen in hospital settings and c * Corresponding author. E-mail address: islahi.sana@gmail.com (S. Islahi). ommunity settings worldwide. 1 It can cause variety of infections including skin infections, joint infections, urinary infections, to pneumonia to septicaemia. 2 Methicillin Resistant Staphylococcus aureus (MRSA) is an emerging multidrug resistant bacteria worldwide, prevalence r anging from 4.6% to 54.4%. 3–6 MRSA grouped under HA MRSA (Healthcare associated methicillin resistant Staphylococcus aureus) and CA MRSA (Community acquired methicillin resistant Staphylococcus aureus). HA MRSA is a pathogen usually acquired during prolonged or frequent hospitalizations and CA MRSA usually affects healthy people, transmission of pathogen occur within community. MRSA is also accountable for outbreak https://doi.org/10.18231/j.ijmmtd.2019.043 2581-4753/© 2019 Innovative Publication, All rights reserved. 188