Open Access Research Article Analytical & Bioanalytical Techniques J o u r n a l o f A n a l y t i c a l & B i o a n a l y t i c a l T e c h n i q u e s ISSN: 2155-9872 Hamd et al., J Anal Bioanal Tech 2016, 7:1 http://dx.doi.org/10.4172/2155-9872.1000294 Volume 7 • Issue 1 • 1000294 J Anal Bioanal Tech ISSN: 2155-9872 JABT, an open access journal Keywords: Non-aqueous titration; Loratadine; Acetous perchloric acid; Acetous crystal violet; Some pharmaceutical preparations Introduction H1-antihistamines antagonists were classified into six groups; alkylamines, monoethanolamines, ethylenediamines, phenothiazines, piperazines, and piperidines [1]. Loratadine (LOR, Figure 1), chosen for the current study, an 4-(8-Chloro-5,6-dihydro-11H-benzo[5,6] cyclohepta[1,2-b]pyridine-11-ylidene)-1-piperidinecarboxylic acid ethyl ester [2,3], crystals from acetonitrile with melting point 134 to 136°C, soluble in alcohol (1 in 10); insoluble in water with a dissociation constant (pKa) 5.0. LOR is belonged to a non-sedating peripheral histamine H1 receptor antagonist, a last generation of H1-antihistamine drugs used to treat allergies, marketed for its non- sedating properties [4]. e aim of the present study was firstly, to find out a rapid method which could be conveniently used for the determination of the percentage purity of a weak bases and insoluble drugs such as LOR (pK a =5.0) in raw material and also in pharmaceutical preparations. Secondly, to discover again the old-fashion, robust, accurate and precise technique of non-aqueous titration, specially the quantitative determination in the concentration range of the developed procedure. A review outlined the previously reported quantitative volumetric and instrumentation methods for determination of LOR and other antihistaminic antagonists analogues was prepared by El-Kommos et al. [1]. e chemical reaction and titrimetric variables were studied and optimized on the basis of lower determined concentrations, temperature, and reagents consumption, and the results obtained by the proposed titrimetric method were comparable with those obtained by an instrumental reported method [5]. Experimental Materials LOR tablets, suspensions (were claimed to contain 5 and 10 mg respectively for tablets and suspensions) and pure LOR standard drug were kindly supplied by Medical Union Pharmaceuticals (MUP, Cairo, Egypt). Glycerin incorporated-LOR nanoparticles were prepared in the laboratory of pharmaceutics department, Faculty of Pharmacy, Al Azhar University (Assuit, Egypt). Glacial acetic acid (CH 3 COOH), perchloric acid (HClO 4 , 70% w/v) and crystal violet powder were obtained from El Nasr Chemical Co., (Abu Zaabal, Egypt). All chemicals used through the study were of analytical reagent grade. Methods Preparation of perchloric acid and crystal violet solution: *Corresponding author: Ahmed A. H. Abdellatif, Department of Pharmaceutics and Industrial Pharmacy, Faculty of Pharmacy, Al-Azhar University, Assuit 71524, Egypt, Tel: +201016660069; E-mail: ahmed.a.h.abdellatif@azhar.edu.eg Received December 11, 2015; Accepted December 28, 2015; Published January 26, 2016 Citation: Hamd MAE, Ali R, Abdellatif AAH (2016) Non-Aqueous Titrimetric Assay for Determination of Loratadine in Pharmaceutical Preparations. J Anal Bioanal Tech 7: 294. doi:10.4172/2155-9872.1000294 Copyright: © 2016 Hamd MAE, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Abstract Non-aqueous titrations is the most titrimetric procedure used in pharmacopoeial assays and serves a double purpose, as it suitable for the titration of weak acids and bases and provides a solvent in which organic compounds are soluble. A simple, cost-effective, rapid, and precise method for determination of loratadine (LOR) in pure and pharmaceutical dosage forms was developed. The method based on non-aqueous titration using acetous perchloric acid as a titrant and acetous crystal violet as indicator. All measurements are carried out by running simultaneous blank determinations and the final titer values are subtracted from blank to get actual amount of acetous perchloric acid equivalent to LOR. The method was successfully applied for determination of LOR in pure and some pharmaceutical preparations. The percentage recoveries of the proposed method were ranged from 84.8 ± 0.1% to 100.9 ± 0.1%. The method showed an acceptable precision and accuracy as it able to determine LOR in presence of the different excipients present in some commercial preparations without any significant interference. Non-Aqueous Titrimetric Assay for Determination of Loratadine in Pharmaceutical Preparations Mohamed A El Hamd 1 , Ramadan Ali 1 and Ahmed A H Abdellatif 2 * 1 Department of Analytical Chemistry, Faculty of Pharmacy, Al-Azhar University, Assiut 71524, Egypt 2 Department of Pharmaceutics and Industrial Pharmacy, Faculty of Pharmacy, Al-Azhar University, Assiut 71524, Egypt N O CH 3 O N Cl Figure 1: Chemical structure of LOR.