International Journal of Research & Review (www.ijrrjournal.com) 448 Vol.6; Issue: 8; August 2019 International Journal of Research and Review www.ijrrjournal.com E-ISSN: 2349-9788; P-ISSN: 2454-2237 Research Paper Effect of Smoking on Follicle-Stimulating Hormone, Luteinizing Hormone and Testosterone in Men Hendri Devita Department of Vocational Midwifery, Baiturrahmah University, West Sumatera Indonesia ABSTRACT Background: Smoking has a negative impact on the reproductive health. The aim of the study was determine effect of smoking on follicle-stimulating hormone (FSH), luteinizing hormone (LH) and testosterone in men. Methods: The study was conducted using a cross sectional study design. The populations in this study were all active smoker and non smoker in Baiturrahmah University with sample size 50 respondents. Sampling technique with consecutive sampling. FSH, LH and testosterone examination in Biochemistry Laboratory of Andalas University. Data analysis used independent sample T test. A two-tailed P-value of <0.05 was considered statistically significant. All data were analysed using SPSS 21.0 program. Results: The results of the study found there were mean differences in FSH level between active smoker and non-smoker were 3.857±1.493 mIU/ml and 4.977±2.083 mIU/ml (p<0.05). LH level showed were 4.984±2.237 mIU/ml and 5.500±2.623 mIU/ml between active smoker and non-smoker (p>0.05). While there were mean differences in testosterone level between active smoker and non- smoker were 15.393±2.782 nmol/l and 20.836±6.360 nmol/l (p<0.05). Conclusion: This analysis confirmed there were effect of FSH and testoterone level between active smoker and non-smoker. Keywords: FSH, LH, Smoking, Testosterone INTRODUCTION Smoking and complications are important social and health problems in all countries. [1] World Health Organization (WHO) estimates that in 2020 smoking- related diseases will become a major health problem in many countries. [2] According to WHO 2008 Indonesia has the third largest number of smokers in the world after China and India. [3] Smoking has a negative impact on the reproductive health of men and women. Cigarettes contain a lot of chemicals. Nicotine is one of the main components of the particulate phase of tobacco combustion. It has been found to be very dangerous for the human body, its absorption through the respiratory tract, oral mucosa and skin, while 80-90% are metabolized by the liver, kidneys and lungs. [4] Nicotine and its metabolites (cotinine) have been found in serum, urine, saliva and milk, and have recently been found in semen plasma in smokers exposed to tobacco smoke. [5] Various hormones including sex steroid hormones can change, due to the effects of nicotine including the axis of the hypothalamus-pituitary-gonad, and metabolism of steroid sex hormones. The prostate and seminal vesicle function can also be influenced by nicotine in cigarettes. [6] Nicotine can affect the work of the central nervous system. [7] Gonadotropin Releasing Hormone (GnRH) in the hypothalamus stimulates the Anterior Hipofise to produce Follicle Stimulating