Corresponding author’s email: agoes_soegianto@fst.unair.ac.id Eco. Env. & Cons. 26 (April Suppl. Issue) : 2020; pp. (S1-S6) Copyright@ EM International ISSN 0971–765X Pathogenic food-borne bacteria in Shellfish and shrimp from the largest traditional seafood market in Surabaya, Indonesia Sin War Naw 13 , Putu Angga Wiradana 1 , Agoes Soegianto 2 * and Aondohemba Samuel Nege 14 1 Fisheries and Marine Biotechnology Study Program, Faculty of Fisheries and Marine, Universitas Airlangga, Jalan Mulyosari, Surabaya 60113, East Java, Indonesia 2 Department of Biology, Faculty of Science and Technology, Universitas Airlangga, Kampus C, Jalan Mulyorejo, Surabaya, East Java 60115, Indonesia 3 Department of Chemistry, Mandalay University, Mandalay City, Myanmar. 4 Department of Fisheries and Aquaculture, Federal University of Agriculture, P.M.B 2373 Makurdi, Nigeria (Received 27 September, 2019; Accepted 10 January, 2020) ABSTRACT The study assessed the levels of Escherichia coli, Salmonella, and Vibrio cholerae in seafood (Anadaragranosa, Pernaviridis, Anadara antiquata, Penaeus monodon, Litopenaeus vannamei) collected from the main traditional seafood market (Pasar Pabean), Surabaya, Indonesia. The aim of the study was to determine and evaluate the level of bacterial contamination if any, found in shellfish and shrimp sold at Pabean market. The total number of Escherichia coli in green mussel (Pernaviridis) samples was about 21,500 CFU/mL and the lowest was recorded in tiger prawn samples (Penaeus monodon) which was 2,800 CFU/mL. The highest Salmonella concentration was obtained from blood clam (Anadara granosa) samples which contained 470 CFU/mL however it was not present in shrimp samples. The highest concentration of Vibrio cholerae was obtained from the antique ark (Anadara antiquata) samples and was about 120 CFU/mL but was absent in green mussels samples, tiger prawn, and vannamei shrimp. In all, it can be concluded that the concentration of Escherichia coli obtained from all the seafood samples in this research exceeds the threshold for human consumption. All the shellfish samples contained Salmonella but Vibrio cholera was only present in two species including blood clam and antique ark. Key words: Seafood contamination, Bio-monitoring, Safety standards, Pathogenic bacteria Introduction The Fisheries sector has an important role in meet- ing human nutritional needs. As an important source of animal protein, aquaculture is rapidly in- creasing globally to meet increasing demand and cover for animal protein deficiency (Alkhunni et al., 2017). For example, according to Iwamoto et al., (2010), the consumption of seafood in the United States has increased in the past decades as an aver- age American recently consumed approximately 16.5 pounds of seafood yearly, compared to the 10- 12 pounds consumed in the 1980s (NOAA, 2005). Similar to other nations, the level of national fish consumption has been on a yearly rise in Indonesia (Ministry of Maritime Affairs and Fisheries of the