37 International Journal for Modern Trends in Science and Technology Biosynthesis of Silver Nanoparticles through Biomass of Fungus Aspergillus niger and their Antibacterial Potential Pushpendra Pratap Singh 1 | Sarika Chaturvedi 2 1 Research Scholar, Amity Institute of Biotechnology, Amity University, Gurugram, Haryana (India) 2 Assistant Professor, Amity Institute of Biotechnology, Amity University, Gurugram, Haryana (India) To Cite this Article Pushpendra Pratap Singh and Sarika Chaturvedi, “Biosynthesis of Silver Nanoparticles through Biomass of Fungus Aspergillus niger and their Antibacterial Potential”, International Journal for Modern Trends in Science and Technology , Vol. 06, Issue 06, June 2020, pp.:37-42; https://doi.org/10.46501/IJMTST060609 Article Info Received on 28-April-2020, Revised on 20-May-2020, Accepted on 25-May-2020, Published on 03-June-2020. Extracellular biosynthesis of silver nanoparticles by fungus Aspergillus niger which was isolated from waste water is being reported in the present paper. The production of silver nanoparticles was evidenced by UV-Vis spectrum, showing the absorbance between 260 to 400 nm. The nanoparticles characterized by Scanning Electron Microscopy exhibited silver nanoparticles with diameter of 25nm to 75nm. Energy Dispersive X-ray analysis reveals strong signals in the silver region and confirms the formation of the silver nanoparticles. The Fourier Transform Infrared Spectroscopy study confirmed that the A. niger mycomass has the ability to perform both reduction and capping functions on the silver nanoparticles. Compound Microscopy confirms the self-assembling property of silver nanoparticles. The silver nanoparticles showed remarkable antibacterial activity against Lactobacillus plantarum, Lactobacillus delbrueckii and Bacillus subtilis bacterial strains. Reduction of silver ions is an extracellular and rapid process; this information may lead to the development of easy protocols for biosynthesis of the silver nanoparticles. Antibacterial activity of silver nanoparticles is important for the development of effective antibacterial agent against those bacteria who are showing resistance against antibiotic drugs which are available in market. KEYWORDS: A. niger, Silver nanoparticles, Antibacterial, Biological Method. Copyright © 2014-2020 International Journal for Modern Trends in Science and Technology DOI: https://doi.org/10.46501/IJMTST060609 I. INTRODUCTION From recent years, nanotechnology playing a very important role in the science and technology, when the significance of nanoparticles was discovered, their application also increased. Nanoparticles have unique electrical, optical and biological properties, these properties makes it applicable in catalysis, agriculture, bio sensing, imaging, drug delivery, nano device fabrication and in medicine [1]. In the past few years, there has been an increasing interest in silver nanoparticles (SNP’s) on account of the antimicrobial properties that they exhibit [2]. Silver nanoparticles are projected as future generation antimicrobial agents [3]. ABSTRACT Available online at: http://www.ijmtst.com/vol6issue06.html International Journal for Modern Trends in Science and Technology ISSN: 2455-3778 :: Volume: 06, Issue No: 06, June 2020