Int. J. Adv. Res. Biol. Sci. (2020). 7(2): XX–XX 1 International Journal of Advanced Research in Biological Sciences ISSN: 2348-8069 www.ijarbs.com DOI: 10.22192/ijarbs Coden: IJARQG(USA) Volume 7, Issue 2 -2020 Research Article Phytochemical and Antibacterial Activity of Acacia nolitica Seed against Gram Positive Bacteria Isolated from Nasal Swab of Patients Attending University of Maiduguri Teaching Hospital, Borno State, Nigeria 1 Mohammed M. Mohammed, 1 Musa John, 2 Abdulbasit H. Yakubu and 3 Halima Nasir 1 Department of Microbiology, Faculty of Science, University of Maiduguri, Borno State, Nigeria 2 Departments of Pharmaceutical Chemistry, Faculty of Pharmacy, University of Maiduguri 3 Department of Science Laboratory Technology, Ramat Polytechnic, Maiduguri, Nigeria Abstract Acacia nolitca (Linn.) has an inspiring range of medical uses. This plant contained several compounds among which have medicinal values. This study aimed to examine the antimicrobial activity of the seed extract of Acacia nolitica against gram- positive bacteria isolated from a nasal swab of patients attending the University of Maiduguri Teaching Hospital using different solvent (Methanol, acetone, and distilled water) of different concentration(50μl, 75μl, and 100μl). The results obtained revealed that the phytochemical analysis of acetone extract of Acacia nilotica showed the presence of Saponins, Alkaloid, Volatile oil, and Terpenoid, while distilled water extract showed the presence of Alkaloid, Volatile oil and Terpenoid. Similarly, methanol extract revealed the presence of Saponins, flavonoid, Alkaloids, Terpenoid and Cardiac glycosides. Staphylococcus aureus and Streptococcus species were isolated from the nasal swab samples of the patients. The sensitivity of the extract against the two isolates revealed that the extract exhibited activity against Staphylococcus aureus while Streptococcus species showed resistance. Therefore, Acacia nolitica may represent a new source of an antimicrobial with a stable biological active component that can establish a scientific base for the use in modern medicine for the treatment of diseases caused by Staphylococcus aureus. Keywords: Phytochemical, Antibacterial Activity, Acacia nolitica, Nasal Swab, In-Patient Introduction Medicinal plants include various types of plants that are used in herbalism for the cure of disease, and many of these plants are very useful in pharmaceutical industries in the production of drugs used today (Bekele et al., 1993). These medicinal plants are considered to be rich sources of ingredients that can be used in drug development and synthesis. Besides, these plants play a critical role in the development of human cultures around the world. Moreover, some plants consider as an important source of nutrition, and as a result of that, these plants recommended for their therapeutic values (Bein, 1996). Medicinal plants have been playing an essential role in the development of human culture. As a source of medicine, medicinal plants have always been at the forefront virtually all cultures of civilizations. Medicinal plants are regarded as sources of traditional medicines and from these plants, many of the modern medicines are produced. DOI: http://dx.doi.org/10.22192/ijarbs.2020.07.02.XXX