_____________________________________________________________________________________________________ *Corresponding author: E-mail: augustineairaodion@yahoo.com; Asian Research Journal of Gynaecology and Obstetrics 3(3): 19-31, 2020; Article no.ARJGO.58343 Frequent Exposure to Air-Freshener Reduces Male Fertility Augustine I. Airaodion 1* , Emmanuel O. Ogbuagu 2 , Festus A. Atiba 3 , Dolapo S. Olawoyin 3 and Oladeji J. Alabi 3 1 Department of Biochemistry, Federal University of Technology, Owerri, Imo State, Nigeria. 2 Department of Pharmacology and Therapeutics, Abia State University, Uturu, Nigeria. 3 Department of Biochemistry, Ladoke Akintola University of Technology, Ogbomoso, Nigeria. Authors’ contributions This work was carried out in collaboration with all authors. Author AIA conceptualized and designed the study and also wrote the manuscript. Author EOO managed the analyses of the study. Author FAA managed the literature searches. Author DSO wrote the protocol while author OJA performed the statistical analysis. All authors read and approved the final manuscript. Article Information Editor(s): (1) Dr. B. Eghon Guzman, Hospital Dr. Sotero del Rio, Chile. Reviewers: (1) Mónica Madrigal Valverde, Costa Rica Institute of Technology, University of Costa Rica, Costa Rica. (2) Abdeldayem Zakaria Mohamed, Alexandria University, Egypt. (3) Alfonso Eduardo Sánchez Riquelme, Universidad de Las Américas, Chile. Complete Peer review History: http://www.sdiarticle4.com/review-history/58343 Received 22 April 2020 Accepted 29 June 2020 Published 11 July 2020 ABSTRACT Background: The use of air-freshener as deodorizer in Nigeria has become worrisome. Some people uses it in the office, car as well as in their homes, thereby constantly being exposed to it without consideration of its adverse effect on their health. Aim: This study is aimed at investigating the effect of air-freshener on sperm quality and male sex hormones. Methodology: Twenty Wistar rats were divided into two groups of ten each and kept in different rooms. Rats in group 1 were not exposed to any substance while those in group 2 were exposed to ‘sunlight’ air-freshener by inhalation for 8 hours daily for 28 days. Throughout the experiment, all animals were fed ad libitum with standard feed and drinking water. At the end of the experiment, rats were sacrificed after an overnight fast under diethyl ether as anesthesia. Blood samples were collected via cardiac puncture. The internal organs were exposed. Testes and cauda epididymis were removed and kept in sterilized watched glass. Sperm quality and sex hormones were determined using standard methods. Original Research Article