Citation: Taleuzzaman M, Ali S, Gilani SJ, Imam SS and Hafeez A. Ultra Performance Liquid Chromatography (UPLC) - A Review. Austin J Anal Pharm Chem. 2015; 2(6): 1056. Austin J Anal Pharm Chem - Volume 2 Issue 6 - 2015 ISSN : 2381-8913 | www.austinpublishinggroup.com Taleuzzaman et al. © All rights are reserved Austin Journal of Analytical and Pharmaceutical Chemistry Open Access Abstract UPLC is a modern technique which gives a new direction for liquid chromatography. UPLC refers to ultra performance liquid chromatography, which enhance mainly in three areas: “speed, resolution and sensitivity. Ultra performance liquid chromatography (UPLC) applicable for particle less than 2μm in diameter to acquire better resolution, speed, and sensitivity compared with high-performance liquid chromatography (HPLC). In twenty frst centenary pharmaceutical industries are focusing for new ways to in economy and shorten time for development of drugs. UPLC analysis at the mean time gives the better quality of their products and analytical laboratories are not exception in this trend. The separation and quantifcation in UPLC is done under very high pressure (up to 100M Pa). As compare to HPLC, under high pressure it is observed that not any negative infuence on analytical column and also other components like time and solvent consumption is less in UPLC. Keywords: Ultra performance liquid chromatography; High separation effciency; Cost effective; High pressure Comparison between UPLC and HPLC Principles are the same but not the performance Te principles of UPLC are same principle as HPLC, the basic diference is in designer of the column material particle size which less than 2-μm. Which make a big deference in performance and to maximize the advantages of these columns, creating a powerful, robust and reliable solution? Te familiar design of UPLC H-class’s Quaternary Solvent Manager (QSM) and Sample Manager (SM- FTN), with fow-through needle design, gives all the fexibility and usability of your current HPLC while still achieving the highly efcient separations that only UPLC can provide [6-9] (Table 1). To improve the UPLC effciency following measures need to be performed 1. By employing high temperature which reduce the viscosity of mobile phase and ultimately fow rate if high. Signifcantly back pressure is reduced. 2. Te unique feature of UPLC analysis is interconnected skeletons and interconnected fow paths (through-pores) which are found in monolithic columns make UPLC technique diferent from HPLC. In UPLC chromatogram it is found that better resolution and separation are found as compared to HPLC along with perform more sensitive analysis, reduce consumption of solvent and has high speed of analysis [8-10]. Small Particle Chemistry Te ideal of the Van Deemter equation cannot be completed without smaller particles than those traditionally used in HPLC. Te Van Deemter equation infuence by particles size, so the scientist focus on the design and development of sub-2 µm particles is a signifcant challenge, and researchers have been active in this area for some time Introduction High performance liquid chromatography (HPLC) has proven to one of the most and predominant technology used in analytical laboratories for the analysis of drugs worldwide during the past 30- plus years [1,2]. One of the basic concerns for the growth of this technique is the packing material which efects the separations. In this separation mechanism the principal apply is Van Deemeter equation, with which any student of chromatography is intimately familiar. H=A+B/v +Cv Te above equation is an empirical formula that describes the relationship between linear velocity (fow rate) and plate height (HETP, or column efciency). And, since particle size is one of the variables, a Van Deemter curve can be used to investigate chromatographic performance. Where A, B and C are constants and v is the linear velocity, the carrier gas fow rate. A= Eddy mixing B =Axial difusion C=Solute’s mass transfer Te A term is independent of velocity and represents “eddy” mixing. It is smallest when the packed column particles are small and uniform. Te B term represents axial difusion or the natural difusion tendency of molecules. Tis efect is diminished at high fow rates and so this term is divided by v. Te C term is due to kinetic resistance to equilibrium in the separation process. Te kinetic resistance is the time lag involved in moving from the gas phase to the packing stationary phase and back again. Te greater the fow of gas, the more a molecule on the packing tends to lag behind molecules in the mobile phase. Tus this term is proportional to v [3-5]. Review Article Ultra Performance Liquid Chromatography (UPLC) - A Review Taleuzzaman M*, Ali S, Gilani SJ, Imam SS and Hafeez A Glocal School of Pharmacy,Glocal University, Saharanpur, 247121, U.P, India *Corresponding author: Mohamad Taleuzzaman, Glocal School of Pharmacy, Glocal University, Saharanpur, India Received: December 13, 2015; Accepted: December 29, 2015; Published: December 31, 2015