Archive of Science & Technology 1 (2020) 75 - 87 R. E. Bodunrinde et al. / Archive of Science & Technology 1 (2020) 75 - 87 75 Synergistic Antibacterial Activity of Hibiscus sabdariffa, Honey and Ciprofloxacin on Selected Uropathogens Ruth Ebunoluwa Bodunrinde 1* ; Muftau Kolawole Oladunmoye 1 ; Michael Tosin Bayode 1 ; Charles Oluwaseun Adetunji 2 and Paul Oladimeji Gabriel 1 . 1 Department of Microbiology, Federal University of Technology Akure, (FUTA), Nigeria. 2 Department of Microbiology, Edo University Iyambo, Edo State, Nigeria. ARTICLE INFO ABSTRACT Article history: Received March 2020 Received in revised form May 2020 Accepted June 2020 Available online June 2020 New treatment methods have been sought to alleviate resistance by uropathogens. The aqueous extract of H. sabdariffa calyx was subjected to gas chromatography-mass spectrophotometry, Fourier transform infrared assays and phytochemical screening following standard methods. In-vitro interactive activity of the H. sabdariffa, honey and ciprofloxacin was conducted by Agar diffusion method. Among the various compounds revealed by GC-MS, the presence of 9-Octadecanoic acid (Z) methyl ester in the aqueous extract validates the antioxidant, anti-inflammatory, anticancer and antimicrobial properties of H. sabdariffa calyx. Fourier transform infrared spectrum of the extract and honey shows the functional groups present at different peaks. Phytochemicals revealed in H. sabdariffa calyx and honey include tannin, phenol, saponin, steroids, terpernoids, flavonoid and glycoside while, alkaloids and phlobatannin were absent. The antibacterial assay showed the efficacy of both plant extract and honey on clinical and typed bacterial strains. H. sabdariffa extract had its minimum inhibitory concentration at 12.5 mg/ml with highest (15.33±0.33) zone against Pseudomonas aeruginosa while honey was only effective at 100% with inhibition zones ranging from 9.54±0.88 – 14.6±0.33 on all tested organisms. The result of the interactive combination showed a synergistic reaction of aqueous extract of H. sabdariffa at higher concentration and lower concentrations of honey and ciprofloxacin giving the highest zone of inhibition (42.00±1.52 mm) on Escherichia coli ATCC 25922. This study has validated the folkloric use of H. sabdariffa, honey and ciprofloxacin to show an overall increase in the activity of their combination against uropathogens which has proved to safeguard the public health of persons with suspected urinary tract infections. Keywords: Antibacterial Combination Hibiscus sabdariffa ethno-medicine uropathogens Synergy. . *Corresponding author. E-mail address: ruthmus2017@gmail.com https://doi.org/10.xxx. 0189-3548 © June 2020 PCU. All rights reserved.