Umayra et al (2020): Immunology of annona muricata January 2020 Vol. 23 (IIb) ©Annals of Tropical Medicine & Public Health S4393 Immunological Role of Annona muricata (Dietary Supplement of gravula) Against Cypermethrin's Toxic Effects in Female Rats Ali Nayyef Umayra 1* , Mohammed Jasim Qasim 2 , Abdul Kareem Salman Al-Yasari 3 and Mohammed Hasan Dakheel 4 1 Department of Environmental Health, Al-Qasim Green University, Iraq 2 Basic science Department, College of Nursing, Misan University, Iraq 3 Department of Microbiology, Al-Qasim Green University, Iraq 4 Public Health Department, Veterinary Medicine, Al-Qasim Green University, Iraq *Corresponding author: skyoneskytwomef@gmail.com Abstract The current research was conducted to investigate the toxic effects of Cypermethrin (CYP) on female rats and the immunological response with or without the oral administration of Graviola (Dietary Supplement) by the use gavager. Sixteen adults female rats aged 60-65 days divided equally into four groups; the first group was orally gavagede with 1 ml of sterile normal saline as a control group, the second group was orally gavagede with 14.5mg/kg B.W. of cypermethrin, while the third group was orally gavaged with 2.5mg/ml of graviola.eWhereas the fourth group was gavaged with 14.5mg/kg of B.W. of cypermethrine plus 2.5mg/ml of graviola. On day 30 the cellulare and humorale immune responses were examinede for all groupse, blood samples were gathered for phagocytic activity measurement and serum was separated by ELISA method for measuring IgG titer.The mean value of phagocytic indices in First, Second, Third and Fourth groups as follow: the first group was (22±3.83) as a control group, the second group was (13.6±2.54) that treated with cypermethrine, the third group was (89±1.82) that treated with graviolae and the fourth group that treated with cypermethrin+graviolae was (68.6±2.21); The current studye showed that the graviolaehad a marked increase in the cellular immune response through the high increase of phagocytic indices due to Toll-like receptor 4 (TLR4) activation. The result of this study showed that the mean value of phagocytic indices in rats that gavaged with CYP, decreased significantly (p<0.05) to 13.6±2.54 as compared to that gavaged with normal saline (control group), which was 22±3.83. The mean value of phagocytic indices in rats that gavaged with both CYP and Graviolae. Increased significantly (p<0.05) to 68.6±2.21 as compared to that gavagede with normal saline (control group), which was 22±3.83. And the group that gavaged with Graviola increased significantly (p<0.05) to (89±1.82) as compared with the control group. Whereas rats that gavagedewith Graviola. Resulted in a further significant (p<0.05) increase to 89±1.82.The result of ELIZA test expressed high value of antibody titers against Graviolae4.86±0.68 g/ml as compared with values of 1.96±0.09 g/ml against cypermethrine. Key Words: Cypermethrin, Graviola, Rats, humoral immune immunity How to cite this article: Umayra AN, Qasim MJ, et al (2020): Immunological role of annona muricata (Dietary supplement of gravula) against cypermethrin’s toxic effects in female rats, Ann Trop Med & Public Health; 23(IIb): S439. DOIhttp://doi.org/10.36295/ASRO.2020.23216 Introduction Cypermethrin (CYP) has been approved as the common name for (RS)—cyano-3- phenoxybenzyl (1RS,3RS;1RS,3SR)-3-(2,2-dichlorovinyl)-2-,2- dimethylcyclopropanecarboxylate by the International Organization for Standardization CYP is a synthetic pyrethroid that is used topically to manage ticks, fleas, moth and blowflies (EMEA,2004).Alfacypermethrine (α−CYP) (two of the four cypermethrine cisomers) is the most powerful CYP and is widely used in farming, cattle and domestic ectoparasitese to safeguard human health (Gilbert et al ,1989; Mueller et al., 2006).