Available online www.jocpr.com Journal of Chemical and Pharmaceutical Research, 2017, 9(5):120-128 Research Article ISSN : 0975-7384 CODEN(USA) : JCPRC5 120 Growth, Characterization and Antibacterial Study of Sulfanilamide Doped by 4-Aminobenzoic Acid C Muthuselvi * , I Selvi and S Pandiarajan Department of Physics, Devanga Arts College, Aruppukottai, Tamil Nadu, India _____________________________________________________________________________ ABSTRACT To enhance the antibacterial activity of toxic drug compound sulfanilamide, it is doped by non-toxic compound 4- aminobenzoic acid by slow evaporation method. The powder XRD analysis shows that the doped crystal has both parent phases. The FT-IR and FT-Raman spectra of doped compound reveals the presence of functional groups of sulfanilamide and 4-aminobenzoic acid. The optical property of doped crystal is analyzed by UV-Vis-NIR spectroscopy. The morphology and elemental composition study confirms the presence of 4-aminobenzoic acid in the crystal lattice of sulfanilamide crystal. The antimicrobial activities of pure and doped crystals were tested against the five different micro-organisms by disc diffusion method. This result reveals that the doped crystal has more antibacterial activity than the pure sulfanilamide crystal. Keywords: Sulfanilamide; 4-aminobenzoic acid; Antibacterial activity; Vibrational spectra; Optical analyzes; SEM with EDX _____________________________________________________________________________ INTRODUCTION The antibacterial drug compound of sulfanilamide is used as an active agent to breakdown the prontosil in the human body [1-3]. This prontosil is inhibiting the growth of streptococci in mice [4-7]. The nontoxic drug of 4- aminobenzoic acid (PABA) is also called vitamin B x which is also used to make various anesthetics drugs. In micro- organism the sulfanilamide’s activity is overcome by PABA due to their structural similarity [8]. The sulfa drug is toxic to all cells which undergo the rapid cancer cell division. To improve the medicinal effect and reducing toxicity of the drug, it is used only their derivatives as a potential drug [8,9]. The chemical modification of sulfanilamide drug gives the broader antibacterial activity and different pharmacological actions [10]. Due to these interesting reports, attempt was made to grow the single crystals of sulfanilamide doped by PABA by slow evaporation method. The grown crystal is characterized by the powder XRD, FT-IR, FT-Raman, UV-Visible spectroscopy and SEM with EDX. These studies and antimicrobial activity techniques lead to confirm the incorporation of PABA into sulfanilamide. MATERIALS AND METHODS Materials The raw materials used for this crystallization are sulfanilamide, ethanol, 4-aminobenzoic acid, double distilled water purchased from Merck, India.