© 2020 |Published by Scholars Middle East Publishers, Dubai, United Arab Emirates 98 Saudi Journal of Pathology and Microbiology Abbreviated Key Title: Saudi J Pathol Microbiol ISSN 2518-3362 (Print) |ISSN 2518-3370 (Online) Scholars Middle East Publishers, Dubai, United Arab Emirates Journal homepage: https://saudijournals.com/sjpm Original Research Article Molecular Identification and Isolation of Antimicrobial, Phytochemical, Antioxidant Properties of Different Solvent Extracts from Wild Mushrooms PGS Manjusha * Department of Microbiology, Researcher, Shoolini University of Biotechnology and Management Sciences, Solan, Himachal Pradesh-173212, India DOI: 10.36348/sjpm.2020.v05i02.011 | Received: 14.02.2020 | Accepted: 21.02.2020 | Published: 23.02.2020 *Corresponding author: PGS Manjusha Abstract The main objective of the present research work was to identify the unknown wild mushrooms based on morphological characteristics and DNA based sequencing by using universal primers ITS 1 and ITS 4 which were collected from Andhra Pradesh and Telangana. To test the potential of various extracts of mushrooms by using three different solvents (Methanol, Hot Water and Hexane). To elucidate antimicrobial properties, qualitative and quantitative estimation of phytochemical properties and antioxidant activity was determined by DPPH free radical scavenging activity. Then, IC 50 values were determined. The four isolates were identified on the basis of morphological and molecular characterization was identified as Trametes versicolor, Daedaleopsis confragosa, Schizophyllum communae and Tyromyces chioneus. Three different solvents had been shown good antimicrobial activity against all pathogenic microorganisms. The hot water extract of T. versicolor exhibited more amount of phenol content (11.13±0.081) and ascorbic acid content (9.62±0.086). Methanol extract of S. communae showed high amount of flavonoids content (12.58±0.138). Methanol extract of T. chioneus exhibited more amount of β-carotene (3.54±0.010) and hot water extract of D. confragosa exhibited more amount of lycopene content (2.29±0.012). The DPPH free radical scavenging activity and IC 50 values was much more exhibited by methanol extracts then followed by hot water extracts and hexane extracts of wild mushrooms they all have exhibited stronger antioxidant activity between (40-50 μg/ml). This comprehensive analysis demonstrates that these mushrooms having a viable source of natural antioxidants that can utilized for functional foods, nutraceutical’s applications with beneficial biological activities and implementation of new drug discovery. Keywords: Antimicrobial, Antioxidant, DNA isolation, Mushrooms and Phytochemical. Copyright @ 2020: This is an open-access article distributed under the terms of the Creative Commons Attribution license which permits unrestricted use, distribution, and reproduction in any medium for non-commercial use (NonCommercial, or CC-BY-NC) provided the original author and sources are credited. ABBREVATIONS DNA – Deoxyribonucleic acid DMSO – Di methyoxy sulfoxide DPPH - 1, 1-diphenyl -2-picrylhydrazyl MEA – Malt Extract Agar MHA – Muller Hinton Agar MHB – Muller Hinton Broth PCR – Polymerase Chain Reaction Highlights of the Paper  The four isolates of Wild mushrooms are Trametes versicolor, Daedaleopsis confragosa, Schizophyllum communae and Tyromyces chioneus has shown good antimicrobial activity against pathogenic microorganisms.  Also, they have exhibited good potential of antioxidant properties.  Molecular identification is done for these species, therefore DNA is extracted by liquid nitrogen method and sequences are submitted to Gene Bank further they provided with accession numbers and Phylogenetic Tree was constructed.  Hence, comprehensive analysis demonstrates that these mushrooms having a viable source of natural antioxidants that can utilized as functional foods, nutraceutical’s applications with beneficial biological activities and implementation of new drug discovery, if further investigation is processed.