Downloaded from www.microbiologyresearch.org by IP: 54.70.40.11 On: Thu, 04 Apr 2019 02:48:35 Marinoscillum luteum sp. nov., isolated from marine sediment In-Tae Cha, 1 Soo-Je Park, 1 So-Jeong Kim, 1 Jong-Geol Kim, 1 Man-Young Jung, 1 Kee-Sun Shin, 2 Kae Kyoung Kwon, 3 Sung-Hyun Yang, 3 Yong-Seok Seo 4 and Sung-Keun Rhee 1 Correspondence Sung-Keun Rhee rhees@chungbuk.ac.kr 1 Department of Microbiology, Chungbuk National University, 12 Gaesin-dong, Cheongju 361-763, Republic of Korea 2 Biological Resources Center, KRIBB, Daejeon 305-806, Republic of Korea 3 Marine Biotechnology Research Center, Korea Ocean Research and Development Institute, PO Box 29, Ansan 425-600, Republic of Korea 4 Department of Earth and Environmental Sciences, Chungbuk National University, 12 Gaesin-dong, Cheongju 361-763, Republic of Korea A novel strain, designated SJP7 T , was isolated from sediment of the Tofua Arc of the Tonga Trench. The 16S rRNA gene sequence of the isolate showed the highest similarity to that of Marinoscillum pacificum KCCM 42325 T (95.9 %). Phylogenetic analysis revealed that the isolate formed a distinct phyletic line with Marinoscillum pacificum KCCM 42325 T and Marinoscillum furvescens LMG 13023 T within the family Cytophagaceae. Cells of strain SJP7 T were Gram- stain-negative and appeared as long rods that were motile by gliding. Growth was observed at 15–40 6C (optimum, 30 6C), at pH 5.5–9.0 (optimum, pH 7.5–8.0) and in the presence of 0.5– 7.0 % (w/v) NaCl (optimum, 2.5–3 %). The major respiratory quinone was MK-7. The dominant fatty acids were summed feature 3 (comprising C 16 : 1 v7c and/or C 16 : 1 v6c), iso-C 15 : 0 and C 16 : 1 v5c. The DNA G+C content was 43.5 mol%. These properties support the affiliation of strain SJP7 T with the genus Marinoscillum. Further phenotypic differentiation of strain SJP7 T from other species of the genus Marinoscillum was indicated by the results of physiological and biochemical tests. On the basis of evidence from our polyphasic taxonomic study, strain SJP7 T represents a novel species of the genus Marinoscillum, for which the name Marinoscillum luteum sp. nov. is proposed. The type strain of Marinoscillum luteum is SJP7 T (5KCTC 23939 T 5NCAIM B02491 T ). Micro-organisms belonging to the phylum Bacteroidetes (previously known as the Cytophaga–Flavobacterium– Bacteroides group) are widely distributed in many envir- onments and play important roles in (polymeric) organic carbon degradation. Marine bacteria of the phylum Bacteroidetes have been frequently isolated during micro- organism screenings using organic-rich media. However, the taxonomic and physiological properties of the marine bacterial strains of the phylum Bacteroidetes have been poorly investigated (Nedashkovskaya et al., 2003). ‘[Microscilla] furvescens’ was originally proposed by Lewin (1969) and Lewin & Lounsbery (1969) to accommodate a Gram-negative, heterotrophic, motile by gliding, strictly aerobic, oxidase-, catalase- and alkaline phosphatase- positive bacterium with rod-shaped or filamentous cells (10 to .100 mm) that lacks flexirubin-type pigments, isolated from marine sands. Recently, based on a polyphasic taxonomic investigation of a novel bacterial strain, MRN461 T , isolated from a marine sponge, Seo et al. (2009) proposed Marinoscillum pacificum gen. nov., sp. nov., in the family Cytophagaceae together with transfer of ‘[Microscilla] furvescens’ to the new genus as Marinoscillum furvescens. Thus, the genus Marinoscillum is represented by two marine bacterial strains isolated from marine sands and sponges, respectively. Here, we report on a polyphasic taxonomic characterization of Marinoscillum-like bacterial strain SJP7 T , which was isolated from sediment of the Tofua Arc of the Tonga Trench. A novel bacterial strain, SJP7 T , was isolated from marine sediment of the Tofua Arc of the Tonga Trench Abbreviation: ASW, artificial seawater. The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of strain SJP7 T is HM161878. One supplementary figure and one supplementary table are available with the online version of this paper. International Journal of Systematic and Evolutionary Microbiology (2013), 63, 3475–3480 DOI 10.1099/ijs.0.050450-0 050450 G 2013 IUMS Printed in Great Britain 3475