ASIAN JOURNAL OF PHARMACOGNOSY Research paper Asian J. Pharmacogn 4(3): 30-36 © 2020, Asian Society of Pharmacognosy. All Rights Reserved. eISSN-0128-1119 30 Comparative phytochemical analysis and antioxidant activities of two herbal powdered drugs used in the treatment of uterine fibroids Kaumadi Karunagoda *1 , Pathirage Kamal Perera 2 , Hemantha Senanayake 3, Liyanage Dona Ashanthi Menuka Arawwawala 4 1 Department of Prasutitantra Kaumarabhritya, Institute of Indigenous Medicine, University of Colombo, Rajagiriya, Sri Lanka 2 Department of DravyagunaVignana, Institute of Indigenous Medicine, University of Colombo, Rajagiriya, Sri Lanka 3 Department of Obstetrics and Gynecology, Faculty of Medicine, University of Colombo, Sri Lanka 4 Industrial Technology Institute, Bauddhaloka Mawatha, Colombo 7, Sri Lanka * For correspondence: kaumadik@gmail.com _______________________________________ Abstract: Nigella sativa Linn (Family: Ranunculaceae) and Anethum sowa Linn (Family: Apiaceae) are commonly used herbs in Ayurveda medicine from ancient times highlighting its’ use in the treatment of uterine fibroids throughout the island in Sri Lanka. Uterine fibroids are the most common benign tumor of the female reproductive tract and occur in about 25% of all women of reproductive age and up to 30 – 40% of women over age 40. N. sativa and A. sowa are used in Sri Lanka for the treatment of uterine fibroids and other common gynecological disorders from ancient days. The phytochemical analysis was carried out by Thin Layer Chromatography and Gas Chromatography analysis. Besides, DPPH scavenging ability, total phenol, and total flavonoid contents were compared. The TLC fingerprints of N. sativa and A. sowa were prominent and can be used as standards for the authentication of these plants. The DPPH free radical scavenging activity was significantly higher in A. sowa than that of N. sativa. Moreover, total polyphenol and flavonoid contents were significantly higher in A. sowa than that of N. sativa. A. sowa showed comparatively more antioxidant activities than that of N. sativa and may use for treating uterine fibroids due to the presence of higher polyphenols and flavonoid types of compounds. © 2020, Asian Society of Pharmacognosy. All Rights Reserved. Keywords: Phytochemical Analysis, Antioxidant Activities, Nigella sativa L., Anethum sowa L. _______________________________________ INTRODUCTION Uterine fibroid is seen during the reproductive life of a female irrespective of the age, which may result in various menstrual problems such as dysmenorrhea, menorrhagia, and irregular periods, by disturbing anatomical as well as physiological integrity. Medical management of this problem is popular based on the Ayurveda system of medicine. Some of these Ayurveda medicines are used single ingredient medicinal plant materials as drugs including Saraca asoca, Emblica officinalis, (Gautam, et.