ISSN: 0975 -8542 Journal of Global Pharma Technology Available Online at www.jgpt.co.in RESEARCH ARTICLE ©2009-2018, JGPT. All Rights Reserved 1 Probiotic Potential of Lactobacillus Plantarum with the Cell Adhesion Properties Abhinandan Patil 1 , John Disouza 1 , Shivaji Pawar 1, 2,* 1 Centre for Interdisciplinary Research, D. Y. Patil University, Kolhapur, (MS). India. 2 Research and Incubation centre, Anekant Education Society, TCC Baramati, (MS). India. * Corresponding Authors Email: shpawar1946@gmail.com Abstract The uses of microbes as functional food maintain health and prevent many disease and disorder. The isolation and research of new strains of probiotic strains especially lactobacilli proven to be useful to satisfy the increasing demand of the population. In the current study, the probiotic potential of Lactobacillus strains isolated from sheep milk was investigated. The Lactobacillus strains were identified and evaluated for tolerance against gastric acidity and bile toxicity, along with the adhesion to HT-29 cells. The study proved the antimicrobial activities and antibiotic susceptibility. Survival of the strains through the host intestine was examined by the 12 week Wister rat feeding and faecal analysis trial. The in-vivo trials not only proved the adhesion but also the survival of the Lactobacillus plantarum MCC 3595 inside the intestinal lumen of the host. Thus, the isolated strain can act as the functional food by the further clinical investigation. Introduction Probiotics are the living microorganisms provide the health benefits when present in sufficient colony forming units inside the host gastrointestinal tract [1, 17]. The probiotic is a term used based upon its efficacy and safety to host and used as functional food for human use [2, 23]. The isolation of the good probiotic strain is been reported by several authors [20]. The strains pass the gradually regarded as safe (GRAS) guidelines are recommended for human use [24]. The isolation of the good probiotic strain is been reported by several authors [22]. The strains pass the (GRAS) guidelines are recommended for human use [24]. One of the criteria for using the probiotic as the formulation is its ability of adhesion to gastrointestinal mucosal surfaces. It should have the antibiotic resistance and probiotics potentially against the pathogenic strains of microorganisms affecting the host immunosystem [3, 25]. The most isolated strains are considered as probiotics are the lactic acid bacteria belonging to the family of Lactobacillus. Most of Lactobacillus strains are non-pathogenic and inhabitants of human gastrointestinal tract maintaining the intestinal microbial ecosystem [4, 20]. Lactobacilli are found effective against the multiplication of enteropathogens and they consistently produce the several antimicrobial compounds [5, 6]. The research related to the novel probiotic strains isolated from the milk source with its utility satisfies the increasing demand of the functional products [7, 10]. The current study deals with the isolation of lactobacilli from the sheep milk with its probiotic characterization and evidence-based study using in-vivo animal model. Materials and Methods Bacterial Strains and Culture Conditions The lactobacilli strain isolated from the sheep milk was used in this study [18, 19]. The strains were studied for cultural, morphological and physiological characteristics along with the sugar fermentation test [9, 21]. PH and Bile Tolerance The Lactobacillus strains were analyzed for their ability to resist to variable pH condition and bile salt concentration. The pH of the gastric acid value is from 1.5–5.0 for a period