BEPLS [Spl. Issue 2] 2016 At 60% wax concentration, the general m mean for shoot length was higher in the genotypes had recorded significant mean G 8, G 12 and G 13penetrate their roots th root length and shoot length was higher the concentrations. The genotypes G 5 a concentration. Three genotypes, G5, G6 penetration ability and tolerance agains Key words: Onion, drought, wax layers, Bulletin of Environment, Pharm Bull. Env. Pharmacol. Life Sci.[Spl. Iss ©2016 Academy for Environment and L Online ISSN 2277-1808 Journal’s URL:http://www.bepls.com CODEN: BEPLAD Global Impact Factor 0.876 Universal Impact Factor 0.9804 http://doi-ds.org/doilink/05.2016-13 Effect of Wax Sub Stress in Assistant Faculty INTRODUCTION Onion (Allium cepa var.aggregatum crop cultivated all over the world. flavonoids in many countries, specific 2008). Drought is a worldwide prob varying degrees of drought. Water de drought, which is one of the most l (Szilgyi, 2003; Hirt and Shinozaki, 2 plant. However, damaging effects of stages such as germination, seedling s al., 2010). Root penetration ability (R growth is difficult to study, especiall developed by Taylor and Gardner (19 the identification of onion genotypes glycol (PEG), a series of polymers th water stress artificially (Larher et al potential (Govindaraj et al., 2010). germination rate, root length, shoot l MATERIALS AND METHODS The experiment was carried out in th Annamalai University, Annamalai n North latitude and 79˚41’ East longit 20 genotypes of diverse origin were are given in Table 1. a) Root penetration ability through Materials • 20 genotypes of onion, listed in 106 | P a g e ©20 ABSTRACT mean for root length was higher in the genotype G 6 (9.85 c e genotype G 14 (15.23 cm). Out of twenty genotypes evalua n value for root length and shoot length respectively. The ge hrough wax layer. At 65% and 70% wax concentration, the g in the genotype G 6. The genotype G 12 and G 13 does not g and G 6 penetrate their roots in 65% wax concentration bu 6 and G8 could be considered as drought tolerant genotyp st PEG-6000. PEG – 6000 macology and Life Sciences sue 2] 2016: 106-111 Life Sciences, India 3212626/ OPEN ACCESS bstrates And Peg Induced Dr n Onion (Allium cepa var.aggregatum) R. Elangaimannan t Professor in Genetics and Plant Breeding, y of Agriculture, Annamalai University Email: relangai74@gmail.com m, 2n =2x= 16) belongs to the family Alliaceae, is an im It has also been shown to be one of the major sou cally, for its flavonol, quercetin and quercetin derivate blem where a major proportion of agricultural land i eficit, extreme temperatures and low atmospheric hu limiting factor for better plant performance and hig 2003). Water stress affects almost every developmen f this stress was more noted when it coincided with v shoot length, root length and flowering (Rauf, 2008; K RPA) is an important character of onion against droug ly in the field. An advantage of the thin wax-layer tech 960). The thin wax-layer technique has been applied s capable of penetrating hard soils (Babu et al., 2001 hat vary from viscous liquids to waxy solids has been l., 1993). PEG induced osmotic stress is found to red An increase in concentration of PEG-6000, resulted length and seed vigor in certain crop plants (Khodarah he orchard of the Department of Horticulture, Faculty nagar, Tamil nadu. The experiment site Annamalai na tude and at an altitude of ± 5.79 m above the mean sea e used for this study. The details of the genotypes use h waxlayers n Table 1. 016 AELS, INDIA cm). The general ated nine and six enotypes G 5, G 6, general mean for germinate at both ut not at 70% wax pes based on root rought mportant spice urces of dietary es (Roldan et al., is affected with umidity leads to gher crop yield ntal stage of the various growth Khayatnezhad et ght stress. Root hnique was first d successfully in 1). Polyethylene used to induce duce cell water d a decrease in hmpour, 2011). y of Agriculture, agar is at 11˚24’ a level. A total of ed in this study