Research Article Effectiveness of Aqueous Extract of Marine Baitworm Marphysa moribidii Idris, Hutchings and Arshad, 2014 (Annelida, Polychaeta), on Acute Wound Healing Using Sprague Dawley Rats Hannah Syahirah Rapi, 1 Nor ‘Awatif Che Soh, 1 Nurul Shahirah Mohd Azam, 1 M. Maulidiani , 1 Suvik Assaw, 2 Mohd Nizam Haron, 3 Abdul Manaf Ali, 4 Izwandy Idris , 5 and Wan Iryani Wan Ismail 1,6,7 1 Cell Signaling and Biotechnology Research Group (CeSBTech), Faculty of Science and Marine Environment, Universiti Malaysia Terengganu, 21030 Kuala Nerus, Terengganu, Malaysia 2 Faculty of Science and Marine Environment, Universiti Malaysia Terengganu, 21030 Kuala Nerus, Terengganu, Malaysia 3 School of Animal Science, Faculty of Bioresources and Food Industry, Universiti Sultan Zainal Abidin, 22200 Besut, Terengganu, Malaysia 4 School of Agriculture Science and Biotechnology, Faculty of Bioresources and Food Industry, Universiti Sultan Zainal Abidin, 22200 Besut, Terengganu, Malaysia 5 South China Sea Repository and Reference Centre, Institute of Oceanography and Environment (INOS), Universiti Malaysia Terengganu, 21030 Kuala Nerus, Terengganu, Malaysia 6 Biological Security and Sustainability (BioSeS) Research Group, Faculty of Science and Marine Environment, Universiti Malaysia Terengganu, 21030 Kuala Nerus, Terengganu, Malaysia 7 Institute of Tropical Aquaculture and Fisheries (Akuatrop), Universiti Malaysia Terengganu, 21030 Kuala Nerus, Terengganu, Malaysia Correspondence should be addressed to Wan Iryani Wan Ismail; waniryani@gmail.com Received 22 June 2020; Revised 14 September 2020; Accepted 24 October 2020; Published 25 November 2020 Academic Editor: Yaw Duah Boakye Copyright©2020HannahSyahirahRapietal.isisanopenaccessarticledistributedundertheCreativeCommonsAttributionLicense, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Wound healing is a well-coordinated process that restores skin integrity upon injury. However, some wound treatment poses harmful effects on the skin, which delay the normal wound healing process. Marphysa moribidii, a marine baitworm or polychaete, represents unique ability to regenerate posterior segment after injury, which may be beneficial in the wound healing treatment. e effectiveness of the polychaete as wound healing treatment was discovered through skin irritation, microbial testing, animal wound model, and chemical identifications. ree polychaete extracts (PE) emulsifying ointment (0.1%, 0.5%, and 1.0%) were topically applied to the full thickness wound model once daily for 14 days. Interestingly, PE 1.0% revealed the most rapid wound healing effects as compared to other treatments, including gamat (sea cucumber) oil (15% w/v) and acriflavine (0.1% w/v). Histopathological analysis using Masson’s trichrome staining further confirms that PE treated wound exhibited minimal scar, high collagen deposition, and the emergence of neovascularisation. e extract also displayed a minimum inhibitory con- centration (MIC) of 0.4 g/ml against Escherichia coli and absence of skin irritation, infectious bacteria, and heavy metals from the extract. Moreover, chemical compounds such as alkaloid, flavonoid, amino acids, and organic acid were detected in M. moribidii extracts, which could contribute to wound healing activity. In conclusion, this study further justifies the beneficial use of polychaete in treating wound healing and could be developed as a novel bioactive agent in nutraceuticals and pharmaceutical drugs. Hindawi Evidence-Based Complementary and Alternative Medicine Volume 2020, Article ID 1408926, 15 pages https://doi.org/10.1155/2020/1408926