~ 147 ~ International Journal of Clinical Obstetrics and Gynaecology 2020; 4(6): 147-150 ISSN (P): 2522-6614 ISSN (E): 2522-6622 © Gynaecology Journal www.gynaecologyjournal.com 2020; 4(6): 147-150 Received: 17-08-2020 Accepted: 03-10-2020 Ramita Malla Tutor, Department of Microbiology, Hind institute of Medical sciences, Safedabad Barabanki, Uttar Pradesh, India Shridevi Metgud Assistant Professor, Department of Obstetrics and Gynaecology, KLE Academy of Higher Education and Research, Jawaharlal Nehru Medical College, Belagavi, Karnataka, India Sharada C Metgud Professor, Department of Microbiology, KLE Academy of Higher Education and Research, Jawaharlal Nehru Medical College, Belagavi, Karnataka, India Corresponding Author: Ramita Malla Tutor, Department of Microbiology, Hind institute of Medical sciences, Safedabad Barabanki, Uttar Pradesh, India Bacteriological profile of Premature rupture of membranes (PROM) in preterm pregnant women: A cross sectional study in Dr. Prabhakar Kore Charitable Hospital, Belagavi, India Ramita Malla, Shridevi Metgud and Sharada C Metgud DOI: https://doi.org/10.33545/gynae.2020.v4.i6c.746 Abstract Background: Premature rupture of membranes is the Preterm Premature rupture of membranes (PPROM) is the rupture of amniotic membranes before the onset of labour prior to 37 weeks gestation. PPROM complicates 3.5% to 5% of pregnancies. There are several risk factors which precipitate PPROM. The commonest risk factors are vaginal infection, short cervix, urinary tract infections. Some common complications seen in Preterm PROM are delivery within one week, cord compression, respiratory distress syndrome, abruptio placenta. Since all patients with PPROM have to be screened for vaginal infections as initiating antibiotics is an important aspect in the management. This study aims to assess the bacteriology involved in PPROM in this centre. Aim & Objectives: To determine bacteria isolated from PPROM and the antibiotic sensitivity of the isolates. Materials and Methods: This is a Cross-sectional study conducted on 60 pregnant women with PPROM who got admitted in labour room of KLE Dr. Prabhakar Kore Charitable Hospital. PPROM was confirmed by nitrazine paper and sterile speculum examination. Under aseptic conditions high vaginal swabs were collected. Result: In this study 57% of the deliveries were through cesarean section. 30% of deliveries included in this study occurred within 24 hours. Bacteria isolated were Enterococcus faecalis (39%), Escherichia coli (28%), Klebsiella pneumonia (13%), Staphylococcus aureus (11%), Proteusmirabilis (6%), Pseudomonas aeruginosa (4%). Imipenem showed (94%) sensitivity to gram negative bacteria isolated and Linezolid showed sensitivity to (77.7%) of Gram-positive isolates compared to other antibiotics. Conclusion: Vaginal infection is an important risk factor for PPROM and preterm labour. Patient with risk need to be monitored closely and correct treatment need to be given to manage PPROM reducing the chances of morbidity and mortality. Keywords: RDS-Respiratory distress syndrome, PPROM-Preterm premature rupture of membrane, VLBW-Very low birth weight, CC – Cord compression Introduction Preterm births contribute to significant maternal and perinatal morbidity and mortality worldwide [14] . The burden of preterm births on the medical management team and the family is huge. 15 million preterm babies are born every year before 37 completed weeks. In most low and middle income countries like India, infections are a leading cause of preterm births. Among the various causes of preterm births, PPROM accounts to about 40% of all preterm births [3] . PPROM is defined as the rupture of membranes before 37 weeks of gestation. It complicates around 8% of all the pregnancies [1] . The etiology of PPROM is multifactorial with cervico- vaginal infections being the most important [4] . Intrauterine infection is also cited to be one of the important cause of PPROM [10] Some common complications seen in preterm PROM are: Delivery within one week (50-75%), respirat0ry distress syndrọme (35%),“ chorioamnionitis(13-60%), abruptio placentae (4-12%),” intrauterine fetal death (1-2%). Management of preterm births presents a significant problem obstetrician and neonatologist [12] . The fetus and amniotic cavity are at risk of infection when the pathogens gain access when: 1. Vaginal flora gets migrated upwards into the amniotic cavity. 2. Placenta infected by hematọgenous spread. 3. Iatrọgenic introduction due to invasive”prọcedures.