4453| International Journal of Pharmaceutical Research | Jul - Dec 2020 | Vol 12 | Supplementary Issue 2 Research Article Chemical characterization of VARUMIN (1 and 2) oral solutions as potential therapeutic beverages VALENTINA PAVLOVA 1 , BILJANA BOGDANOVA POPOV 1 , VESNA. KARAPETKOVSKA HRISTOVA 1* , SYED KHALID MUSTAFA 2 , ATIF ABDULWAHAB A. OYOUNI 3 , MOHAMMAD REHAN AJMAL 4 , M. AYAZ AHMAD 5 , OTHMAN ALZAHRANI 3 , AMR AHMED ELSAYED 6 , MOHAMMAD ALI SHARIATI 7 *1 Faculty of Technological and Technical Sciences – University “St. Kliment Ohridski” Bitola, Department of Biochemical Sciences,”Partizanska” bb 7000 Bitola, Macedonia 2 Department of Chemistry, Faculty of Science, P.O.B. 741, University of Tabuk, 71491, Saudi Arabia 3 Department of Biology, Faculty of Science, P.O.B. 741, University of Tabuk, 71491, Saudi Arabia 4 Department of Biochemistry, Faculty of Science, P.O.B. 741, University of Tabuk, 71491, Saudi Arabia 5 Department of Physics, Faculty of Science, P.O.B. 741, University of Tabuk, 71491, Saudi Arabia 6 Department of Food Science, Faculty of Agriculture, Zagazig University, Egypt 7 Laboratory of Biocontrol and Antimicrobial Resistance, Orel State University named after I.S. Turgenev, Russia *Corresponding Author Email:- v.primavera79@gmail.com Received: 20.07.20, Revised: 04.08.20, Accepted: 10.09.20 ABSTRACT Varumin (1 and 2) is known as commercially produced drugs in the Republic of Macedonia. It contains different herbal medicines such as mistletoe, elecampane, corneltree bark, marigold, yarrow, and cynodon and St John’s wort. In addition, Varumin (1 and 2) has been designed to increase the defending strength (immunity) of different parts of the human body infected by acute and chronic diseases. Since herbal drugs profiles of Varumin (1 and 2), the present study aimed to assessing the chemical profile of potent compounds in order to give a better understanding of possible therapeutical mechanisms in the occurrence of diseases via detecting compounds present in Varumin (1 and 2). The obtained results revealed that the amount of Rutin and Quercetin ranged between 63.04 µg/mL to 64.09 µg/mL and 65.93 µg/mL to 66.26 µg/mL respectively. And, the total flavonoids were found between the range of 1.901 ± 0.08 µg/mL to 2.094 ± 0.08 µg/mL. Finally, the Non Enzymatic Antioxidant properties (NEAOP) have been studied well and the obtained results were in the range of 85.91±1.63 µg/mL to 97.95  0.24 µg/mL. All obtained values were found within good agreement with other workers. Keywords: Rutin, Quercetin, NEAOP, Varumin (1and 2), Flavonoids, St John’s wort INTRODUCTION The Varumin (1and 2) is a generic herbal product which strengthens the human body against different acute and chronic sicknesses of lower grades of immunity such as anemia (Linde K. et al, 2001 & 2002 and also references therein) [1, 2]. This product in solution form is exploited orally as Varumin 1 which impart organs invaded with purgative. Varumin 2 have antimicrobial, anti- hyperglycemic and antiviral properties (G. Dimitrov et. al, 2014) [3]. Varumin 1 contains different ingredients. According to the German Commission E, Aloe (Aloe ferox Mill., Aloe vera Mill., Aloaceae, former Liliaceae) is an agent in treating constipation (Abid Aslam M. et al., 2018) [4]. Mistletoe herb (Visci albi herba, Viscum album L., Loranthaceae) contains Varumin which acts as anti-inflammatory agent as well as in treating malignant tumors approved by Monograph no. 228, 1984 [5]. The drug is considered as a traditional medicine of Balkan region for improving body conditions such as blood pressure (Tucakov 1984) [6]. The propolis, is a conventional medical component used to treat some diseases such as gastrointestinal disorders, dermatitis (Bogdanova Popov et al., 2017) [7]. Inulae helenii radix, Inula helenium L., Asteraceae is a herbs that contains varumin 2 which imparts on respiratory and gastrointestinal diseases as antibacterial, antifungal and anti- tumor agents (Huo et al., 2008) [8]. Another herb rich in varumin 2 is Corni mas cortex, (Cornus mas L., Cornaceae) which enhances regulating activity of abdominal functions (Petkov 1979) [9]. To prevent granulation of skin damages, alleviating abdominal pains and healing body wounds, Marigold flower (Calendulae flos, Calendula ISSN 0975-2366 DOI:https://doi.org/10.31838/ijpr/2020.SP2.579