Research J. Pharm. and Tech. 13(12): December 2020 1 ISSN 0974-3618 (Print) www.rjptonline.org 0974-360X (Online) RESEARCH ARTICLE Preparation, Standardisation and Evaluation of Hypoglycaemic effect of Herbal Formulation containing five Ethnomedicinal plants in Alloxan- Induced Hyperglycemic Wistar Rats Archana Sharma 1 *, Prosanta Pal 2 , Bapi Ray Sarkar 2 , J. P Mohanty 1 , Sonam Bhutia 3 1 Himalayan Pharmacy Institute, Majhitar, Sikkim University, East Sikkim-737136 2 Department of Pharmaceutical Technology, University of North Bengal, West Bengal, India-734013 3 Government Pharmacy College, Government of Sikkim, Sikkim University, Sajong, Rumtek East Sikkim- 737135 *Corresponding Author E-mail: sonamkzbhutia@gmail.com ABSTRACT: The present study was undertaken to evaluate the pharmacognostical parameters, formulation, standardization and hypoglycaemic effect of herbal preparation in alloxan induced diabetic rats. The physicochemical properties of polyherbal finished product were: Specific gravity (1.03 at 25˚C), pH of 10%v/v solution (4.543), solid content (7% w/v), viscosity (4.59 cp), surface tension (43.37 dynes/cm) and refractive index (1.37). The colour (brown), odour (sweet aromatic), and taste (sweet) of polyherbal formulation was satisfactory in physical appearance. The study was also aimed to evaluate the antidiabetic activity of herbal formulation in alloxan induced diabetic rats model. The results of the body weight and blood glucose level of control, diabetic control group, standard group (Glibenclamide 5mg/kg)-standard drug used and two different doses of trial formulation (600mg and 300mg/kg)-summarised in Table No 1 and Table No 2 respectively. The initial body weight of Herbal formulation low dose and high dose were 123.33±5.78 and 185±8.38 respectively and after 11 days of treatment the bodyweight was decreased to 119.33±8.45 and1 70.33±7.79 respectively. Two different doses of the herbal formulation (low dose and high dose) i,e 123.33±5.78 and 185±8.38 blood glucose level were studied in two different groups of animals. Both groups showed a significant decrease of blood glucose level on alloxan induced diabetic rats when compared to control group. The low dose result shows for respective day 3: 5.01±0.21, day 7: 33.23±1.70 and day11: 63.20±2.46 and for high doses for day 3: 6.59±0.34, day 7: 35.26±1.84 and day 11: 65.63±2.46. Both groups showed a significant decrease of blood glucose level on alloxan induced diabetic rats when compared to control group. The present study showed that the investigation of the polyherbal formulation containing five herbs has a potent anti diabetic effect which could be used in the management of diabetes mellitus effectively. KEYWORDS: Anti-hyperglycaemic, Herbal preparation, Ethno medicinal plants, Standardization, Alloxan- Induced anti-diabetic activity. INTRODUCTION: India is one of the most significant nations on the planet as far as floristic decent variety. About 54% of the nation's property is under development of nourishment, fancy and restorative plant crops and roughly 19% area has fluctuating level of woods vegetation spread. Received on 25.04.2020 Modified on 11.06.2020 Accepted on 05.08.2020 © RJPT All right reserved Research J. Pharm. and Tech. 2020; 13(12):. DOI: Land based formative exercises give methods for occupation, wellbeing and open door for business [1] . India has worldwide situation in the field of conventional meds. About 90% home grown crude medications utilized in the assembling of vegetable medications are acquired from the wild source which is restricted. There are around 45,000 plants species in India which are in copious in the locales of Eastern Himalaya, Western Ghats and Andaman and Nicobar Islands. The rich legacy of verdure is because of broadened and differed agro-climatic conditions. The authority recorded plants with restorative potential are 3,000 however