Copyright: © the author(s), publisher and licensee Technoscience Academy. This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial License, which permits unrestricted non- commercial use, distribution, and reproduction in any medium, provided the original work is properly cited International Journal of Scientific Research in Science and Technology Print ISSN: 2395-6011 | Online ISSN: 2395-602X (www.ijsrst.com) doi : https://doi.org/10.32628/IJSRST207567 307 Study of Iron, Inorganic Phosphate, Sodium, Potassium in Serum and its Effects on Reproduction of the Experimental Fish Clarias Batrachus Dr. Amit Kumar University Department of Chemistry, B. R. A. Bihar University, Muzaffarpur, Bihar, India. E-mail: thisisamitsingh@yahoo.co.in Article Info Volume 7, Issue 5 Page Number: 307-314 Publication Issue : September-October-2020 Article History Accepted : 15 Oct 2020 Published : 27 Oct 2020 ABSTRACT Reproduction is a natural phenomenon governed by both the endocrine (intrinsic) and climatic (extrinsic) factors. The intrinsic factors mediate their effects through the hypothalamo-hypophysial-Gonad sub system operating as an organic axis bridging the ecological influence and Gonadal maturation. Temperature, light, rainfall etc. of the habitat and food supply appear to the predominant external factors influencing breeding periodicity, whereas, the condition of the Gonads and their secretions & the general condition of the body are the internal features which influence the physiology & psychology of the breeders to make the target organs responsible to external influences (Malhotra et.al., 1989). Among the climatic factors, temperature and/or day length (Photoperiod) are generally recognized as the most important cues in the timing of gametogenesis in fish species of temperate zone (Gillet et.al., 1978) but in sub-tropical region, when seasonal variations in the day length & temperature are relatively small both affects Gonadal recrudescence. In this paper we determine the Iron, Inorganic Phosphate, Sodium, Potassium in Serum and its Effects on Reproduction of the Experimental Fish Clarias Batrachus. Keywords: Protein, Glucose, Albumin, Globulin, Fish, Clarias Batrachus (Linn). I. INTRODUCTION In tropical & sub-tropical regions, the peak spawning activity in the fish is often associated with flood, rainfall & lunar cycles (Schwassmann, 1971, 1980; Liley, 1980), but it is still not clear that which one of the terminal reproductive events i.e. oocyte maturation, ovulation or oviposition is enhanced by rainfall or whether spermiation is involved. Temperature dependent seasonal variations of the electrophoretic protein patterns were demonstrated by Schlotfeldt (1975), whereas, other workers (Haider, 1970b; Perrier et al. 1973a) could not observed any change in Salmo guairdneri. Increasing water temperature enhances the metabolic activities in most of the fish species,