Journal of Animal Husbandry and Dairy Science Volume 3, Issue 2, 2019, PP 18-24 ISSN 2637-5354 Journal of Animal Husbandry and Dairy Science V3 ●I2 ●2019 18 Deep Sequence and Microarray Techniques of Salmonella Enterica Serovar Typhimuriumin Horses Amira Saad Helal Hassenin* Assistant professor, faculty of Veterinary Medicine, Zagazig University, Egypt *Corresponding Author: Amira Saad Helal Hassenin, Assistant professor, faculty of Veterinary Medicine, Zagazig University, Egypt, Email: amirasaad@zu.edu.eg INTRODUCTION Importance of these bacteria of the Salmonella genus is widespread in nature. They can be detected in many cold and warm-blooded animals across the globe. In numerous countries today they are the most important bacterial diarrhea-causing pathogens in man. As they are mostly transmitted from animals to man through consumption of foods of animal origin, salmonellae are classified as zoonosis ("zoonosis" is the scientific term for all diseases which can be transmitted by pathogens from animals to man).They transmit genetic material (DNA) in resistant strains. ETIOLOGY Smith et al (1978) Reported that Salmonellosis, a serious infectious disease of equids, caused by different serovars of Salmonella enterica subspecies enterica, often precipitates as fatal septicemia and severe diarrhea in foals and colitis/typhilitis in equids of all ages. Salmonellae are the most frequent causes of acute diarrhoea in horses and the incidence seems to be increasing (Powell et al., 1988, Traub- Dargatz et al., 1990). Michael et al (2005) Indicated that Salmonellae may be a primary cause of infection in foals under 6 months old. The serovar abortus equi (groupB with O ANTIGEN epitopes 4and 12) belonging to salmonella enterica subspp entrica is acause of equine paratyphyoid although salmonella typhimurium has been reported (Gall et al 2006). Niwa et al (2009) Reported that the equine affected with different seovar of salmonella but the most sever one is the salmonella typhimurium. EPIDEMIOLOGY Horses recovering from acute salmonellosis may act as a source of environmental contamination with Salmonella spp for a variable period of time (Palmer and Benson, 1985) monitored 81 horses recovering from acute enteritis for a period of 30 to 718 days. Although 31 horses were lost to follow-up, the median period of fecal shedding of Salmonella spp was <30 days. In another population, 2 of 50 horses on a farm exposed to 43 horses recovering from acute salmonellosis transiently shed Salmonella spp. Outbreaks of equine salmonellosis are generally associated with a high population density of horses such as occurs on large horse farms and in equine veterinary hospitals Horses admitted to hospitals are more susceptible to Salmonella infection because of multiple stresses, which may include predisposing illness, transportation and ABSTRACT My research can detect this transmitted gene that usually cause of antibiotics resistance to help control this type of salmonella stain by treatment and control in animal as well as human Sero typing has been the core of public health monitoring of Salmonella infections for over 50 years. Now, Diagnosis use DNA testing to further divide each serotype into more subtypes and to detect more outbreaks. With the next generation of sequencing technology, advancements continue as the laboratory can find information about the species, serovar, and subtype of bacteria in just one test. Availability of resources will determine the extent of research carried out by me, versus research conducted at collaborating labs. Keywords: salmonella, horses, gene, antibiotics, serotype