Copper and zinc in rhizospheric soil of wild plants growing in long-term acid vineyard soils. Insights on availability and metal remediation C. Campillo-Cora a , D. Fernández-Calviño a, ⁎, P. Pérez-Rodríguez a , M.J. Fernández-Sanjurjo b , A. Núñez-Delgado b , E. Álvarez-Rodríguez b , M. Arias-Estévez a , J.C. Nóvoa-Muñoz a a Department of Plant Biology and Soil Sciences, Faculty of Sciences, University of Vigo, 32004 Ourense, Spain b Department of Soil Science and Agricultural Chemistry, Engineering Polytechnic School, University of Santiago de Compostela, 27002 Lugo, Spain HIGHLIGHTS • Available Cu levels were higher in rhizo- sphere than in bulk soil. • Cu and Zn increases in the rhizosphere were associated to increases in organic matter. • Zn was accumulated mainly in the aerial biomass of wild plants. • Cu accumulation in roots or aerial bio- mass was dependent on wild plant spe- cies. • Low Cu translocation in D. sanguinalis, P. hieracioides, S. viridis, T. barbata GRAPHICAL ABSTRACT abstract article info Article history: Received 11 December 2018 Received in revised form 21 February 2019 Accepted 20 March 2019 Available online 22 March 2019 Editor: Yaoyu Zhou Total and available Cu and Zn levels were assessed in plant biomass, as well as in two rhizosphere fractions (tightly adhering rhizosphere (TAR), and loosely adhering rhizosphere (LAR)), in wild plants species from vine- yard soils. Both TAR and LAR fractions were enriched in total Cu and Zn (1.7 and 1.6 times, respectively), and in available Cu and Zn (2.2 and 19.5 times, respectively), with the former being significantly higher for TAR than for LAR fractions. Mean values for total Cu accumulation in root and aerial biomass of the studied wild plants were 84 and 66 mg kg -1 , respectively, being 57 and 79 mg kg -1 for Zn. No correlations were found among metal contents in plant biomass and available Cu and Zn concentrations in the rhizosphere fractions. Translocation factor (TF) values for Zn (range 1.0–3.5) indicate preferential accumulation in the aerial biomass in all the studied wild plants. On the contrary, TF for Cu shows a greater variability, depending on plant species, and ranging from 0.2 to 5.9. Regarding bioaccumulation factor (BAF), ranges were 0.03–0.27 and 0.13–0.58, for Cu and Zn, respectively. Results suggest that D. sanguinalis, P. hieracioides, S. viridis, and T. barbata could be useful for Cu remediation in the studied soils, by means of phytostabilization processes. © 2019 Elsevier B.V. All rights reserved. Keywords: Availability Cu and Zn pollution Phytostabilization Rhizosphere Vineyard soils Science of the Total Environment 672 (2019) 389–399 ⁎ Corresponding author. E-mail address: davidfc@uvigo.es (D. Fernández-Calviño). 1. Introduction The intensive and continuous application of Cu-base fungicides to vineyards soils since mid XIX century had led to a significant Cu https://doi.org/10.1016/j.scitotenv.2019.03.301 0048-9697/© 2019 Elsevier B.V. All rights reserved. Contents lists available at ScienceDirect Science of the Total Environment journal homepage: www.elsevier.com/locate/scitotenv