Copyright: © the author(s), publisher and licensee Technoscience Academy. This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial License, which permits unrestricted non- commercial use, distribution, and reproduction in any medium, provided the original work is properly cited International Journal of Scientific Research in Chemistry (IJSRCH) © 2020 IJSRCH | Volume 5 | Issue 6 | ISSN : 2456-8457 56 Toxicоlogical Evaluation of Eclipta alba using Brine Shrimp (Artemia salina L.) Model Akshay R.Yadav*, Dr. Shrinivas K. Mohite 1-2 Department of Pharmaceutical Chemistry, Rajarambapu College of Pharmacy, Kasegaon, Dist- Sangli, Maharashtra, India *Corresponding author E-mail:- akshayyadav24197@gmail.com Article Info Volume 5, Issue 6 Page Number: 56-62 Publication Issue : November-December-2020 Article History Accepted : 15 Nov 2020 Published : 30 Nov 2020 ABSTRACT Brine shrimp lethality assay is the most useful tool for tracking the biological behavior of different plant species. This method is useful in advance for the toxicity evaluation of plant extracts. Eclipta alba leaves have long been in use in the Indian Local Medicine System for their antioxidant and anti-inflammatory properties. The toxicity of Eclipta alba herb extracts using this assay was determined within a concentration range of 1mg/ml, 100μg/ml, 10μg/ml and 1μg/ml of the herbal extract being tested. Most studies of the toxicity with a Brine Shrimp lethality test measure toxicity after 24 hours of exposure to the examined sample. The Brine shrimp lethality assay is a perfect method for assessing the toxic potential of plant extracts. Keywords: Eclipta alba, Brine shrimp lethality assay, Toxicity testing, Artemia salina I. INTRODUCTION Now-a-days brine shrimp lethality assay is commonly used to check the bioactive chemicals cytotoxic effect. This is a preliminary screening of plant extracts for toxicity. Subsequently animal model for establishment is recommended 1 . Other top assays at the bench are inhibition of crown gall tumors on potato tuber disks, frond proliferation inhibition in duckweed and yellow fever larvae lethality test. Between them, the lethality test for brine shrimps is the shortest, low cost and effective one 2-3 . The nauplii about 22 mm long, are large enough to observe in a laboratory without high magnification and small enough to hatch in vast amounts without extensive workspace 4 . This is a rapid and thorough test for bioactive compounds of either natural or synthetic origin. It is also a cheap and simple test, since no aseptic techniques are required. It easily uses a large number of species for statistical testing and needs no special equipment, and needs fairly low sample amounts (2-20 mg or less) 5 .This in vivo test has been used successively since its introduction for the bioassay-guide fractionation of active cytotoxic and antitumor agents. The expression of poisonous toxicity indicates the state of adverse effects arising from the contact between the toxicant and cell. This interaction is subject to the toxic chemistry and the cell membrane because it may occur in the surface of the cells, the cell body or in the underlying tissues,