International Journal of Forest, Animal and Fisheries Research (IJFAF) ISSN: 2456-8791 [Vol-5, Issue-1, Jan-Feb, 2021] Issue DOI: https://dx.doi.org/10.22161/ijfaf.5.1 Article DOI: https://dx.doi.org/10.22161/ijfaf.5.1.3 www.aipublications.com/ijfaf Page | 18 Accuracy of Various Doses of Sulphate Acid (H 2 SO 4 ) in early Pregnancy Detection in Friesian Holstein Dairy Cows Euis Nia Setiawati 1 , Dadang Mulyadi Saleh 2 , Vony Armelia 2 and Aras Prasetya Nugroho 2 1 Animal Health Training Center, Cinagara Bogor, Indonesia 2 Faculty of Animal Science, Jenderal Soedirman University, Purwokerto, Indonesia Corresponding E-mail: e.niasetiawati@gmail.com Received: 30 Oct 2020; Received in revised form: 15 Jan 2021; Accepted: 30 Jan 2021; Available online: 16 Feb 2021 ©2021 The Author(s). Published by AI Publications. This is an open access article under the CC BY license (https://creativecommons.org/licenses/by/4.0/) Abstract— This study aims to find out the accuracy of doses and time in early detection of pregnancy using sulfuric acid (H2SO4) concentrated in FH dairy cows. A total of 600 urine samples came from 120 FH dairy cows that had been artificially inseminated on the 24th day, the 34th day, the 44th day, the 60th day. Urine samples are taken in the morning from 06.00 – 10.00. Concentrated doses of Sulphate Acid (H2SO4) are divided into 3 including P1 (0.1 ml), P2 (0.5 ml) and P3 (1.0 ml) according to treatment. The observed variables are gas fluorescence in the solution, discoloration of the limes pink solution, and the timing of changes in the solution. To ensure the pregnancy is carried out rectal palpation examination day 60 after artificial insemination. The collected data is analyzed with the Chi-Square test. Chi-Square test analyst results showed that sulphate acid (H2SO4) dose treatment is no different (P >0.05) in detecting early development. The optimal dose of use of Sulphate Acid (H2SO4) concentrated in detecting early production of friesian holstein milk cows of age is achieved at a dose of 0.5 ml with an accuracy of 83.33 % - 95.23 %, which indicated the formation of air bubbles (fluorensce) and pink in the duration of time, with a duration of 9-45 seconds. Conclusion Early pregnancy detection technology method "Concentrated Sulphate Acid (H2SO4) can be used to detect cow pregnancy 24 days after insemination. Keywords— early pregnancy, Sulphate Acid (H2SO4), Rectal Palpation, FH dairy cows. I. INTRODUCTION Dairy cows are one of the mainstay businesses of the livestock sub-sector that is prospective in agribusiness activities. The development of the livestock business has a positive impact on job creation and promises cash income, to motivate farmers to play an active role in agribusiness activities to increase their family income (Kaliky and Hidayat, 2006). Dairy cows are very potential cattle to be developed because it is by the needs of Indonesia today. The success of efforts to increase the productivity of dairy cows depends on the population. Real conditions in smallholder cause slow population development and low milk production, among others, due to high reproductive disorders in female dairy cows. Assessment of reproductive efficiency in dairy cows in the field shows the length of the distance between the mother gave birth to bunting back (Days Open) (Atabany et. al. 2017; One of the causes of Days Open is due to the failure of early detection of domestication in dairy cows after artificial insemination (AI). Pregnancy is a series of immunologic and endocrinological changes in the effort to produce children in the womb from fertilization to normal birth. As the pregnant ages, some conception hormones, especially estrogen and progesterone, increase (Frastantie et al., 2019).